지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 38 - 44 (7page)
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초록· 키워드
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Cisplatin is widely used for various types of cancers. However, its side effects, most notably, renal toxicity often limit its clinical utility. Although previous metabolomic studies reported possible toxicity markers, they used small number of animals and statistical approaches that may not perform best in the presence of intra-group variation. Here, we identified urinary biomarkers associated with renal toxicity induced by cisplatin using NMR-based metabolomics combined with Orthogonal Projections to Latent Structures-Discriminant Analysis (OPLS-DA). Male Sprague-Dawley rats (n=22) were treated with cisplatin (10 mg/kg single dose), and the urines obtained before and after treatment were analyzed by NMR. Multivariable analysis of NMR data presented clear separation between non-treated and treated groups. The OPLS-DA statistical results revealed that 1,3-dimethylurate, taurine, glucose, glycine and branched-chain amino acid (isoleucine, leucine and valine) were significantly elevated in the treated group and that phenylacetylglycine and sarcosine levels were decreased in the treated group. To test the robustness of the approach, we built a prediction model for the toxicity and were able to predict all the unknown samples (n=14) correctly. We believe the proposed NMR-based metabolomics with OPLS-DA approach and the resulting urine markers can be used to augment the currently available blood markers.
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참고문헌 (32)
참고문헌 신청
Arany, I / 2003 / Cisplatin nephrotoxicity / Semin. Nephrol 23:460~464
Boudonck, K. J / 2009 / Discovery of metabolomics biomarkers for early detection of nephrotoxicity / Toxicol. Pathol 37:280~292
Bylesjo, M / 2006 / OPLS discriminant analysis: combining the strengths of PLS-DA and SIMCA classifi cation / J. Chemom 20:341~351
Clayton, T. A. / 2009 / Pharmacometabonomic identifi cation of a signifi cant host-microbiome metabolic interaction affecting human drug metabolism / Proc. Natl. Acad. Sci. USA 106:14728~14733
Clayton, T. A / 2006 / Pharmaco-metabonomic phenotyping and personalized drug treatment / Nature 440:1073~1077
Boudonck, K. J / 2009 / Discovery of metabolomics biomarkers for early detection of nephrotoxicity / Toxicol. Pathol 37:280~292
Bylesjo, M / 2006 / OPLS discriminant analysis: combining the strengths of PLS-DA and SIMCA classifi cation / J. Chemom 20:341~351
Clayton, T. A. / 2009 / Pharmacometabonomic identifi cation of a signifi cant host-microbiome metabolic interaction affecting human drug metabolism / Proc. Natl. Acad. Sci. USA 106:14728~14733
Clayton, T. A / 2006 / Pharmaco-metabonomic phenotyping and personalized drug treatment / Nature 440:1073~1077
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