지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국식품영양과학회 Journal of Food Science and Nutrition Journal of Food Science and Nutrition Vol.15 No.4
- 발행연도
- 2010.12
- 수록면
- 360 - 363 (4page)
이용수
초록· 키워드
오류제보하기
To enhance the expression and secretion of pediocin PA-1 from heterologous bacterial hosts, the promoter and deduced signal sequence (PS) of an α-amylase gene from a Bifidobacterium adolescentis strain was fused with pediocin PA-1 structural and immunity genes (AB) and the resulting functions were evaluated in Escherichia coli. Two recombinant PCR products were created－one with just the deduced signal sequence and one with the sequence plus the Ser and Thr sequences that are the next two amino acids of the signal sequence. These two products, the PSAB (---AQA::KYY---) and PSABST (---AQAST::KYY---), respectively, were inserted into a TA cloning vector (yT&A) and named pPSAB, which was previously reported, and pPSABST. The two recombinant plasmid DNAs were transferred into E. coli JM109 and the transformants displayed antimicrobial activity, where the activity of E. coli JM109 (pPSAB) was stronger than that of E. coli JM109 (pPSABST), indicating that the ST amino acid residues were not necessary for secretion and might have even decreased the antimicrobial activity of recombinant pediocin PA-1.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES
참고문헌 (9)
참고문헌 신청
Rodríguez JM / 2002 / Pediocin PA-1, a wide-spectrum bacteriocin from lactic acid bacteria / Crit Rev Food Sci Nutr (42):91~121
Gálvez A / 2007 / Bacteriocin-based strategies for food biopreservation / Int J Food Microbiol (120):51~70
Sit CS / 2008 / Approaches to the discovery of new antibacterial agents based on bacteriocins / Biochem Cell Biol (86):116~123
Rodríguez JM / 2003 / Heterologous production of bacteriocins by lactic acid bacteria / Int J Food Microbiol (80):101~116
Moon GS / 2006 / Expression and purification of a fusion-typed pediocin PA-1 in Escherichia coli and recovery of biologically active pediocin PA-1 / Int J Food Microbiol (108):136~140
Gálvez A / 2007 / Bacteriocin-based strategies for food biopreservation / Int J Food Microbiol (120):51~70
Sit CS / 2008 / Approaches to the discovery of new antibacterial agents based on bacteriocins / Biochem Cell Biol (86):116~123
Rodríguez JM / 2003 / Heterologous production of bacteriocins by lactic acid bacteria / Int J Food Microbiol (80):101~116
Moon GS / 2006 / Expression and purification of a fusion-typed pediocin PA-1 in Escherichia coli and recovery of biologically active pediocin PA-1 / Int J Food Microbiol (108):136~140
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UCI(KEPA) : I410-ECN-0101-2012-511-003794946