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논문 기본 정보

자료유형
학술저널
저자정보
Hyoung Do Kim (원광대학교) Se Eun Ha (원광대학교) Jea Ran Kang (원광대학교) Jong Kun Park (원광대학교)
저널정보
고려인삼학회 Journal of Ginseng Research Journal of Ginseng Research Vol.34 No.3
발행연도
2010.9
수록면
205 - 211 (7page)

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초록· 키워드

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Korean red ginseng (KRG) has been used worldwide as a traditional medicine for the treatment of various diseases, includ- including cancer. In this study, we determined the effect of KRG on the responses of HaCaT cells to peroxynitrite (ONOO?). Cells treated with ONOO? (2 mM) prior to incubation with control medium for 12 hours displayed reduced viability, as determined using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay (viability about 48% of that of non-treated control cells). When KRG was added to the post-incubation medium, the negative effects of ONOO? on cell viability were significantly reduced. Reverse transcription-polymerase chain reaction analysis indicated that KRG alone did not significantly alter p53 or “growth arrest and DNA damage” (GADD)45 mRNA levels. However, the addition of KRG to the post-incubation medium significantly and dose-dependently reduced levels of p53 and GADD45 mRNA in ONOO?-treated cells. Western blot analyses revealed that incubation with KRG decreased p53 and GADD45 protein levels in ONOO?-treated cells, relative to those in cells incubated with control medium. Collectively, these results suggest that Korean red ginseng extract protects cells against ONOO?-induced genotoxicity by increasing cell viability through modulating the expression of p53 signaling intermediates.

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INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES

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