지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.50 No.5
- 발행연도
- 2009.10
- 수록면
- 461 - 466 (6page)
이용수
초록· 키워드
오류제보하기
The induction of haploid plants by in vitro gynogenesis is a promising practice in onion breeding. For improvement of this technique, the effects of spermidine as a component of induction media on gynogenic embryo production and regeneration of plantlets were studied. The unpollinated flowers from three Iranian long day onion populations, Sefid-e-Yazd, Ghermez-e-Neyshabor and Sefid-e-Qom populations were used as initial explants. Embryo production and regeneration of plantlets increased significantly, when induction media supplemented with a combined treatment of 0.5 mM spermidine, 2 mgㆍL?¹ 2,4-D and 2 mgㆍL?¹ BAP. Addition of spermidine alone had an inhibitory effect and no embryo was produced. The highest embryo generation capacity (1.9%) was obtained in Sefid-e-Qom population which was more responsive than the other two populations. The period of maximum production of embryos was between 59-80 days after culture of flowers on induction media. Addition of spermidine along with 2,4-D and BAP had no adverse effect view of increasing hyperhydricity, but callus rate increased slightly. Ghermez-e-Neyshabor had the highest rate of hyperhydricity and callus rate (5.29%, 2.54%), respectively. Forty percent of the embryos obtained could regenerate whole plantlets and among regenerants, 77.3% were haploid and 13.6% were diploid.
목차
Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
Literature Cited
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