지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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We tried to develop the protocol for embryogenesis and plant regeneration from anther culture of carrot (Daucus carotaL.) genotype ‘S&P2342’. Anthers were cultured on MS medium with B5 vitamins containing different combinations of 2,4-Dand NAA for 18 weeks in the dark. The best induction of callus and embryo was obtained in the medium containing 0.1 mg/L2,4-D and 0.1 mg/L NAA, on which 22.0% callus and 2.0% embryo were induced. When primary embryos induced directly fromanther culture were transferred to the regeneration medium, secondary embryos were initiated from primary embryos after 4weeks of culture and 62.5% converted into plantlets after 8 weeks of culture. The plantlets with true leaves were obtained after12 weeks of culture. When the calli derived from anther culture were transferred to the regeneration medium, 38.8% of the calliproduced primary embryos and plantlets after 8 weeks of culture. The plantlets with 2 or more leaves cultured on the regenerationunder the different light intensity for the growth of in vitro plantlets. The plantlets cultured at 100 μmol･m-2･s-1 showed thehighest growth rate. For the acclimatization, the in vitro plantlets with 4 or more leaves cultivated under the different lightintensity and temperature, respectively. The survival rate and growth of plantlets was best at 15℃ and 100 μmol･m-2･s-1,respectively. The plants were successfully acclimatized and had a normal phenotype. The anther culture system could be usedto prepare the doubled haploid lines as an appropriate breeding material for F1 hybrid breeding program.
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참고문헌 (20)
참고문헌 신청
Alessandro MS / 2007 / Inheritance of vernalization requirement in carrot / J. Am. Soc. Hortic. Sci 132 : 525 ~ 529
Andersen SB / 1990 / Biotechnology in agriculture and forestry, Vol 12. Haploids in crop improvement I / Springer : 393 ~ 402
Flehinghaus T / 1991 / Methodical improvements in rye anther culture / Plant Cell Rep 10 : 397 ~ 400
Gamborg OL / 1968 / Nutrient requirements of suspension cultures of soybean root cell / Exp. Cell Res 50 : 151 ~ 158
Gorecka K / 2005 / The influence of several factors on the efficiency of androgenesis in carrot / J. Appl. Genet 46 : 265 ~ 269
Andersen SB / 1990 / Biotechnology in agriculture and forestry, Vol 12. Haploids in crop improvement I / Springer : 393 ~ 402
Flehinghaus T / 1991 / Methodical improvements in rye anther culture / Plant Cell Rep 10 : 397 ~ 400
Gamborg OL / 1968 / Nutrient requirements of suspension cultures of soybean root cell / Exp. Cell Res 50 : 151 ~ 158
Gorecka K / 2005 / The influence of several factors on the efficiency of androgenesis in carrot / J. Appl. Genet 46 : 265 ~ 269
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