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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.47 No.3
- 발행연도
- 2006.6
- 수록면
- 110 - 116 (7page)
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초록· 키워드
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The aim of this research was to produce and analyze doubled haploid (DH) lines from long-day onion through in vitro gynogenesis. Unopened flower buds were cultured on modified BDS medium containing 1 ㎎ㆍL?¹ benzyladenine and 1 ㎎ㆍL?¹ 2,4-dichlorophenoxyacetic acid. To produce the pollen parents (C line), a total of 11,850 flower buds from ten cultivars were inoculated, giving rise to 103 sprouting embryos (0.87%). The best gynogenic plantlet rates were 2.77 and 2.03% in cvs. Getsurin and Candy, respectively. To produce the maintainer line (B line), a total of 15,150 flower buds from four lines were inoculated, giving rise to 270 sprouting embryos (1.78%). When ploidy level was checked by flow cytometry using 49 regenerants, haploids, mixoploids, and diploids were found to be 24.5, 44.9, and 16.3%, respectively. When bulb characteristics of S1 generation from the DH lines were analyzed, the bulbs had uniform shape as an elliptic or circular type. Genetic heterozygosity of the DH lines were analyzed by randomly amplified polymorphic DNA. The genetic similarity among the S₁ generation was clustered in one group at 0.80 similarity index using a NTSYS program. In addition, the observed and the expected heterozygosity were calculated to be 0.379 and 0.400, respectively.
목차
Abstract
Introduction
Material and Methods
Results and Discussion
Literature Cited
참고문헌 (27)
참고문헌 신청
[학술지(정기간행물)] - / 2004 / Fecund gynogenic lines from onion (Allium cepa L.) 167 : 1055 ~ 1066
[학술지(정기간행물)] - / 1922 / The assortment of chromosome in triploid Daturas 56 : 339 ~ 346
[학술지(정기간행물)] - / 1999 / Variation in gynogenic response among long-day onion 18 : 737 ~ 742
[학술지(정기간행물)] - / 1995 / Studied of gynogenesis in onion Induction procedures and genetic analysis of regenerants 104 : 215 ~ 224
[학술지(정기간행물)] - / 1984 / Efforts for in vitro androgenesis in onion
[학술지(정기간행물)] - / 1922 / The assortment of chromosome in triploid Daturas 56 : 339 ~ 346
[학술지(정기간행물)] - / 1999 / Variation in gynogenic response among long-day onion 18 : 737 ~ 742
[학술지(정기간행물)] - / 1995 / Studied of gynogenesis in onion Induction procedures and genetic analysis of regenerants 104 : 215 ~ 224
[학술지(정기간행물)] - / 1984 / Efforts for in vitro androgenesis in onion
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