지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.3
- 수록면
- 55 - 58 (4page)
이용수
초록· 키워드
오류제보하기
Generally, the direct sequencing through PCR is faster, easier, cheaper, and more practical than clone sequencing. Frequently, standard PCR amplification is usually interpreted by mispriming internal or external regions of the target template. Normally, DNA fragments were eluted from the gel using Gel extraction kit and subjected to direct sequencing or cloning sequencing. Cloning sequencing has often troublesome and needs more time to analyze for many samples. Since touchdown (TD) PCR can generate sufficient and highly specific amplification, it reduces unwanted amplicon generation. Accordingly, TD PCR is a good method for direct sequencing due to amplifying wanted fragment. In plants the 5S-rRNA gene is separated by simple spacers. The 5S-rRNA gene sequence is very well-conserved between plant species while the spacer is species-specific. Therefore, the sequence has been used for phylogenetic studies and species identification. But frequent occurrences of spurious bands caused by complex genomes are encountered in the product spectrum of standard PCR amplification. In conclusion, the TD PCR method can be applied easily to amplify main 5S-rRNA and direct sequencing of panax ginseng cultivars.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGMENTS
REFERENCES
참고문헌 (19)
참고문헌 신청
1. [학술지(정기간행물)] - Rao, V. B. / 1994 / Strategies for direct sequencing of PCR-amplified DNA / PCR Methods Appl. 4 : 15 ~ 23
2. [학술지(정기간행물)] - Bevan, I. S. / 1992 / Sequencing of PCR-amplified DNA / PCR Methods Appl. 1 : 222 ~ 228
3. [학술지(정기간행물)] - Moncany, M. L. / 1993 / Comparison of some procedures to recover the PCR-amplified products for a direct sequencing / Genet Anal Tech Appl. 10 : 24 ~ 26
4. [학술지(정기간행물)] - Clark, J. M. / 1988 / Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases / Nucleic Acids Res. 16 : 9677 ~ 9686
5. [학술지(정기간행물)] - Lohff, C. J. / 1992 / PCR using a thermostable polymerase with 3' to 5' exonuclease activity generates blunt products suitable for direct cloning / Nucleic Acids Res. 20 : 144 ~
2. [학술지(정기간행물)] - Bevan, I. S. / 1992 / Sequencing of PCR-amplified DNA / PCR Methods Appl. 1 : 222 ~ 228
3. [학술지(정기간행물)] - Moncany, M. L. / 1993 / Comparison of some procedures to recover the PCR-amplified products for a direct sequencing / Genet Anal Tech Appl. 10 : 24 ~ 26
4. [학술지(정기간행물)] - Clark, J. M. / 1988 / Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases / Nucleic Acids Res. 16 : 9677 ~ 9686
5. [학술지(정기간행물)] - Lohff, C. J. / 1992 / PCR using a thermostable polymerase with 3' to 5' exonuclease activity generates blunt products suitable for direct cloning / Nucleic Acids Res. 20 : 144 ~
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