메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류

추천
검색

논문 기본 정보

자료유형
학술저널
저자정보
저널정보
대한피부과학회 Annals of Dermatology Annals of Dermatology 제23권 제3호
발행연도
2011.1
수록면
304 - 312 (9page)

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색

초록· 키워드

오류제보하기
Background: Multiplex polymerase chain reaction (PCR)allows more than two target DNA molecules to be amplified with more than two primers. This method is also useful for detecting various other organisms simultaneously within a single test tube, and the scope of its use has been expanding widely in the field of clinical microbiology in recent years. Objective: To assess the value of multiplex PCR in identification of dermatophytes. Methods: Using three speciallydesigned primers which contained the ITS1-2, 18S rRNA,and 28S rRNA regions, three cycles of PCR were performed on 11 standard strains and scales were collected from 73patients with fungal infection. Results: The 11 standard strains were successfully identified with analysis of band patterns of ITS1-2, 18S rRNA, and 28S rRNA, obtained from PCR. Based on this information, the causative organisms in 73 patients with fungal infection were revealed to be T. rubrum in 69 cases, T. menta in 1 case, T. tonsurans in 2cases, and M. gypseum in one case. Conclusion: With three cycles of PCR using three sets of primers, 11 standard strains and the clinical strains from 73 patients with fungal infection were successfully identified. (Ann Dermatol 23(3) 304∼312, 2011)

목차

등록된 정보가 없습니다.

참고문헌 (18)

참고문헌 신청

이 논문의 저자 정보

최근 본 자료

전체보기

댓글(0)

0