지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
플라스미드 pGKV21에는 229-nt single-strand DNA initiation (ssi) signal이 존재한다. Asymmetric PCR 기법으로 합성된 229-nt ssDNA 단편을 이용하여 실제로 RNA polymerase에 의한 priming ability와 protein interaction을 확인하였다. in vitro primer RNA 합성 실험 결과, 229-nt ssDNA 단편은 filamentous M13 phage의 주형 DNA에서와 비슷한 효율로 시발체 RNA를 합성하였으며, 이 반응은 strand-specific하게 이루어졌다. DNase I footprinting과 gel retardation 실험 결과, RNA polymerase와 SSB 단백질은 229-nt ssDNA 단편에 stable interaction을 하며, 시발체 RNA를 합성하였다. 또한, in vivo 조건 하에서 RNA polymerase의 저해제인 rifampicin을 처리하여 세포 내에 ssDNA 중간체가 집적되는 정도를 비교하여 본 결과, 플라스미드 pGKV21은 rifampicin-sensitive RNA polymerase가 상보가닥 합성에 관여 함을 보여 주었다.
목차
Introduction
Materials and Methods
Results and Discussion
Acknowledgment
References
초록
참고문헌 (23)
참고문헌 신청
1. [학술지(정기간행물)] - Arai, K. / 1981 / Mechanism of dnaB protein action. V. Association of dnaB protein, protein n', and other repriming proteins in the primosome of DNA replication / J. Biol. Chem. 256 : 5273 ~ 5280
2. [학술지(정기간행물)] - Bouche, J. P. / 1975 / dnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single stranded coliphage DNA to its duplex replication form / J. Biol. Chem. 250 : 5995 ~ 6001
3. [학술지(정기간행물)] - Bujalowski, W. / 1986 / Escherichia coli single-strand binding protein forms multiple, distinct complexes with single-stranded DNA / Biochemistry 25 : 7799 ~ 7802
4. [학술지(정기간행물)] - Bujalowski, W. / 1988 / Binding mode transitions of Escherichia coli single strand binding protein-single-stranded DNA complexes. Cation, anion, pH, and binding density effects / J. Biol. Chem. 263 : 4629 ~ 4640
5. [학술지(정기간행물)] - Chase, J. W. / 1986 / Single-stranded DNA binding proteins required for DNA replication / Annu. Rev. Biochem. 55 : 130 ~ 136
2. [학술지(정기간행물)] - Bouche, J. P. / 1975 / dnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single stranded coliphage DNA to its duplex replication form / J. Biol. Chem. 250 : 5995 ~ 6001
3. [학술지(정기간행물)] - Bujalowski, W. / 1986 / Escherichia coli single-strand binding protein forms multiple, distinct complexes with single-stranded DNA / Biochemistry 25 : 7799 ~ 7802
4. [학술지(정기간행물)] - Bujalowski, W. / 1988 / Binding mode transitions of Escherichia coli single strand binding protein-single-stranded DNA complexes. Cation, anion, pH, and binding density effects / J. Biol. Chem. 263 : 4629 ~ 4640
5. [학술지(정기간행물)] - Chase, J. W. / 1986 / Single-stranded DNA binding proteins required for DNA replication / Annu. Rev. Biochem. 55 : 130 ~ 136
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