지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.12
- 수록면
- 273 - 278 (6page)
이용수
초록· 키워드
오류제보하기
Stem cells have been the subject of increasing scientific interest because of their utility in numerous biomedical applications. Stem cells are capable of renewing themselves; that is, they can be continuously cultured in an undifferentiated state, giving rise to more specialized cells of the human body. Therefore, stem cells are an important new tools for developing unique, in vitro model systems to test drugs and chemicals and a potential to predict or anticipate toxicity in humans. In the present study, in vitro cultured F3 immortalized human neural stem cell line and in vivo adult Sprague Dawley rats was used to evaluate the cytotoxicity of anticancer drug paclitaxel. In vitro apoptotic activity of paclitaxel was evaluated in F3 cell line by a MTT assay and DAPI test. The cell death was induced with the treatment of 20 nM paclitaxel and chromatin degradation was detected by DAPI staining, which was analyzed by fluorescent microscope. In vivo studies, we also observed nestin immunoreactivity on subventricular zone, which is stem cell rich region in the adult brain of the SD rat. Immunofluorescent staining result shows that pixel intensities of nestin were decreased in a dose dependent manner. These results suggest that paclitaxel is able to induce cytotoxic activity both in F3 neural stem cell line and neural stem cell in SD rat brain.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
Acknowledgements
REFERENCES
참고문헌 (21)
참고문헌 신청
1. [학술지(정기간행물)] - Clarke, DL / 2000 / Generalized potential of adult neural stem cells / Science. 288 : 1660 ~ 1663
2. [학술지(정기간행물)] - Derry, WB / 1995 / Stoichiometric binding of taxol suppresses microtubule dynamics / Biochemistry. 34 : 2203 ~ 2211
3. [학술지(정기간행물)] - Han, G-Z / 2005 / Synergism between the Anticancer Actions of 2-Methoxyestradiol and Microtubule- Disrupting Agents in Human Breast Cancer / Cancer Res. 65 : 387 ~ 393
4. [학술지(정기간행물)] - Long, MH / 1994 / Paclitaxel inhibits progression of mitotic cells to G1 phase by interference with spindle formation without affecting other microtubule functions during anaphase and telophase / Cancer Res. 54 : 4355 ~ 4361
5. [학술지(정기간행물)] - Madiraju, C / 2005 / Tubulin assembly, taxoid site binding, and cellular effects of the microtubule-stabilizing agent dictyostatin / Biochemistry 44
2. [학술지(정기간행물)] - Derry, WB / 1995 / Stoichiometric binding of taxol suppresses microtubule dynamics / Biochemistry. 34 : 2203 ~ 2211
3. [학술지(정기간행물)] - Han, G-Z / 2005 / Synergism between the Anticancer Actions of 2-Methoxyestradiol and Microtubule- Disrupting Agents in Human Breast Cancer / Cancer Res. 65 : 387 ~ 393
4. [학술지(정기간행물)] - Long, MH / 1994 / Paclitaxel inhibits progression of mitotic cells to G1 phase by interference with spindle formation without affecting other microtubule functions during anaphase and telophase / Cancer Res. 54 : 4355 ~ 4361
5. [학술지(정기간행물)] - Madiraju, C / 2005 / Tubulin assembly, taxoid site binding, and cellular effects of the microtubule-stabilizing agent dictyostatin / Biochemistry 44
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UCI(KEPA) : I410-ECN-0101-2010-510-001484562