지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.12
- 수록면
- 263 - 272 (10page)
이용수
초록· 키워드
오류제보하기
Nebulin is a giant actin binding protein (600~900 kDa) which is specific to skeletal muscle. This protein is known to regulate thin filaments length in sarcomere as a molecular template. The C-terminus of nebulin is located in the Z-disc of muscle sarcomere and is bound to other proteins such like myopalladin, titin, archvillin, and desmin. The N-terminus of nebulin binds to tropomodulin at the pointed ends of the thin filaments. In recent research, nebulin not only found in brain but also expressed in heart, stomach, and liver. So, the roles of nebulin in non-muscle tissue have been studied. However, lack of information or studies on nebulin binding proteins and nebulin function in brain are available so far. Therefore, the current study have investigated a novel binding partner of Nebulin C-terminus by using yeast two-hybrid screening with human brain cDNA library. Nebulin C-terminus, containing simple repeats, serine rich and SH3 domain, interacts with osteonectin C-terminal region. The specific interaction of nebulin and osteonectin were confirmed in vitro by using GST pull-down assay and reconfirmed in vivo by using transfected COS-7 cells with EGFP-tagged nebulin and DsRed-tagged osteonectin. Consequently, this study identified SH3 domain in nebulin C-terminus specifically binds to extracellular Ca-binding (EC) domain in osteonectin. Also, nebulin C-terminus fusion protein colocalized with osteonectin EC domain fusion protein in transfected COS-7 cells. The current study found the interaction between nebulin and osteonectin in human brain for the first time and suggested the nebulin in brain may be associated with osteonectin, as a regulator of cell cycle progression and mitosis.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (41)
참고문헌 신청
1. [학술지(정기간행물)] - Bang, ML / 2001 / Myopalladin, a novel 145-kilodalton sarcomeric protein with multiple roles in Z-disc and I-band protein assemblies / J Cell Biol. 153 : 413 ~ 427
2. [학술지(정기간행물)] - Bang,ML / 2002 / Molecular dissection of the interaction of desmin with the C-terminal region of nebulin / J Struct Biol. 137 : 119 ~ 127
3. [학술지(정기간행물)] - Bornstein, P / 1995 / Diversity of function is inherent in matricellular proteins: an appraisal of thrombospondin / J Cell Biol. 130 : 503 ~ 506
4. [학술지(정기간행물)] - Bradshaw, AD / 2001 / SPARC, a matricellular protein that functions in cellular differentiation and tissue response to injury / J Clin Invest. 107 : 1049 ~ 1054
5. [학술지(정기간행물)] - Chen, MJ / 1993 / Nebulin is an actin-binding, length regulating template protein of the thin filaments of skeletal muscle: actin-interaction and confirmation of a two module human nebulin fragment / Biophys J. 64 : 147 ~ 159
2. [학술지(정기간행물)] - Bang,ML / 2002 / Molecular dissection of the interaction of desmin with the C-terminal region of nebulin / J Struct Biol. 137 : 119 ~ 127
3. [학술지(정기간행물)] - Bornstein, P / 1995 / Diversity of function is inherent in matricellular proteins: an appraisal of thrombospondin / J Cell Biol. 130 : 503 ~ 506
4. [학술지(정기간행물)] - Bradshaw, AD / 2001 / SPARC, a matricellular protein that functions in cellular differentiation and tissue response to injury / J Clin Invest. 107 : 1049 ~ 1054
5. [학술지(정기간행물)] - Chen, MJ / 1993 / Nebulin is an actin-binding, length regulating template protein of the thin filaments of skeletal muscle: actin-interaction and confirmation of a two module human nebulin fragment / Biophys J. 64 : 147 ~ 159
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
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Desmin Binding Property of Nebulin Isoforms
대한의생명과학회지
2006 .06
-
Identification of Non-Muscle Nebulin Isoform in Human Brain Library
대한의생명과학회지
2004 .03
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Regulated Expression of Nebulin by Transfection of Green Fluorescent Protein-Tagged Nebulin Fragments in Cultured Chicken Myoblast
대한의생명과학회지
2001 .12
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Identification of a Protein Interacting with Human Nebulin SH3 Domain by Yeast Two-hybrid Screening
대한의생명과학회지
2001 .06
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Nebulin C-terminus Interacts with NCBP51, a New Isoform of RING Finger Protein 125 (RNF125)
대한의생명과학회지
2007 .03
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닭 조직에 따른 Nebulin Isoform 단백질의 특이적 발현
대한의생명과학회지
2000 .09
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Nebulin in Z-discs and Costameres
대한의생명과학회지
2003 .12
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Archvillin C-Terminus-Binding Proteins in Human Skeletal Muscle
대한의생명과학회지
2010 .12
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백서의 실험적 치아이동시 osteonectin의 osteocalcin의 발현
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1998 .10
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Differential Expression of Osteonectin in the Rat Developing Molars
International Journal of Oral Biology
2012 .01
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Knockdown of Archvillin by siRNA Inhibits Myofibril Assembly in Cultured Skeletal Myoblast
대한의생명과학회지
2007 .12
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가토 하악골에서 신연 골형성술시 Osteonectin의 발현
대한구강악안면외과학회지
2004 .01
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성인에서 원위근병증으로 발현된 네말린근병증
Annals of Clinical Neurophysiology
2016 .01
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The Effect of Estrogen Deficiency on Rat Pulpodentinal Complex
Restorative Dentistry and Endodontics
2005 .01
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BAPN으로 유도된 구개파열 백서의 osteonectin발현 양상에 대한 연구
대한치과교정학회지
2000 .08
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MC3T3-E1 조골세포주의 osteonectin과 bone sialoprotein mRNA
대한구강악안면방사선학회지
2004 .01
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Bone Healing Capacity of Demineralized Dentin Matrix Materials in a Mini-pig Cranium Defect
Journal of Korean Dental Science
2012 .06
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하악골 신장술에서 압축자극을 통한 골재생시 TGF-β1, Osteonectin 및 BMP-4의 발현
대한구강악안면외과학회지
2007 .01
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Studies on the Membrane Topology of the (Na, K) ATPase
한국응용약물학회 춘계학술발표논문집
1996 .01
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Molecular interaction of $G{\alpha}16$ subunit G protein with chemoattractant receptors
대한약학회 학술대회
2002 .01
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UCI(KEPA) : I410-ECN-0101-2010-510-001484557