지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 51 - 56 (6page)
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초록· 키워드
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Tooth development involves bud, cap, bell and hard tissue formation stages, each of which is tightly controlled by regulatory molecules. The aim of this study was to identify genes that are differentially expressed during dental hard tissue differentiation. Sprague-Dawley rats at postnatal days 3, 6 and 9 were used in the analysis. Differential display RT-PCR (DD-PCR) was used to screen differentially expressed genes between the 2nd (root formation stage,during mineralization) and 3rd (cap stage, before mineralization)molar germs at postnatal day 9. The DNA detected in the 2nd molar germs showed homology to osteonectin only (GenBank accession no. NM_012656.1). The level of osteonectin mRNA expression was much higher in the 2nd molar germs than in the 3rd molar germs and was found to increase in a time-dependent manner from the early bell stage to the root formation stage in the 2nd molar germs.
The pattern of osteonectin protein expression was consistent with these RT-PCR results. Osteonectin protein was found by immunofluorescent analysis to localize in odontoblasts and preodontoblasts rather than the dentin matrix itself.
Further studies are needed to validate the involvement of osteonectin in mineralization and root formation.
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등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Hansen L / 2007 / A novel nonsense mutation in PAX9 is associated with marked variability in number of missing teeth / Eur J Oral Sci 115 : 330 ~ 333
Jee-Hae Kang / 2011 / The Epithelial-Mesenchymal Transition During Tooth Root Development / International Journal of Oral Biology 36 (3) : 135 ~ 141
Lane TF / 1994 / The biology of SPARC, a protein that modulates cell-matrix interactions / FASEB J 8 (2) : 163 ~ 173
Mann K / 1987 / Solubilization of protein BM-40 from a basement membrane tumor with chelating agents and evidence for its identity with osteonectin and SPARC / FEBS 218 : 16
Mason I.J / 1986 / Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial “culture shock” glycoprotein
Jee-Hae Kang / 2011 / The Epithelial-Mesenchymal Transition During Tooth Root Development / International Journal of Oral Biology 36 (3) : 135 ~ 141
Lane TF / 1994 / The biology of SPARC, a protein that modulates cell-matrix interactions / FASEB J 8 (2) : 163 ~ 173
Mann K / 1987 / Solubilization of protein BM-40 from a basement membrane tumor with chelating agents and evidence for its identity with osteonectin and SPARC / FEBS 218 : 16
Mason I.J / 1986 / Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial “culture shock” glycoprotein
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