메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류

추천
검색
질문

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색
질문

초록· 키워드

오류제보하기
Chicken lymphomas caused by either Marek's disease virus (MDV) or avian leukosis/sarcoma virus (ALSV) were fastidious diseases for the differential diagnosis using ordinary methods including histopathology and PCR assays. Therefore, the aim of this study is to develop DNA chip for the differential diagnosis of Marek's disease (MD) and avian leukosis/sarcoma (ALS) in chickens. Using the primer pairs specific to each serotype of MDV and each subgroup of ALSV, single PCR assays were carried out with DNA extracted from each prototype strain of MDVs and ALSVs and amplified each specific band for prototype strains of MDVs and ALSVs. DNA chip assays were performed with the products by multiplex PCR assays using mixture of primer pairs specific to each MDV serotype and each ALSV subgroup virus. The positive reaction of each serotype of MDV and each subgroup of ALSV was detected on the DNA chip slide. DNA chip assays also detected MDVs even in negative by RT-PCR assays. From these results, the DNA chip assays developed promised accurate and rapid diagnosis for the differentiation of MD and ALS in chickens. This is the first report to detect MDVs and ALSVs by DNA chip assays in the world.

목차

재료 및 방법
결과
고찰
감사의 글
참고문헌

참고문헌 (19)

참고문헌 신청

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

이 논문과 함께 이용한 논문

최근 본 자료

전체보기

댓글(0)

0

UCI(KEPA) : I410-ECN-0101-2009-510-016364190