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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 299 - 306 (8page)
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초록· 키워드
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Assay for the detection and quantification of porcine circovirus type 2 (PCV 2) with the Real-time PCR were developed. TaqMan probe real-time using a set of primer/probe was developed for detection of PCV 2. In this study we applied Real-time PCR assay to 320 samples, collected from pig farms. In 151 of 320 samples, PCV 2 DNA was detected by conventional PCR assay. All samples positive for PCV 2 DNA in conventional PCR assay were also positive in Real-time PCR assay, but 69 of 169 samples that tested
negative for PCV 2 DNA in conventional assay were tested positive in TaqMan probe real-time PCR assay. The test of TaqMan probe real-time PCR resulted in detection and quantification limits of 101 copies per
sample. TaqMan probe real-time PCR assay increased the number of samples in which PCV 2 was detected by 21%. TaqMan probe real-time PCR assay is very efficient method in contrast to the conventinal PCR,
becoming increasingly important method for gene analysis.
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참고문헌 (19)
참고문헌 신청
Brunborg IM / 2004 / Quantitation of porcine circovirus type 2 isolated from serum/plasma and tissue samples of healthy pigs and pigs with postweaning multisystemic wasting s
Cheung AK / 2004 / Identification of essential and non-essential transcription units for protein synthesis, DNA replication and infectious virus production of Porcine circovi
Choi C / 2000 / Porcine postweaning multisystemic wasting sindrome in Korean pig: detection of porcine circovirus 2 infection by immunohistochemistry and polymerase chain rea
Chung WB / 2005 / Real-time PCR for quantitation of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in naturally-infected and challenged pig
Fenaux M / 2002 / Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: Characterization of clinical d
Cheung AK / 2004 / Identification of essential and non-essential transcription units for protein synthesis, DNA replication and infectious virus production of Porcine circovi
Choi C / 2000 / Porcine postweaning multisystemic wasting sindrome in Korean pig: detection of porcine circovirus 2 infection by immunohistochemistry and polymerase chain rea
Chung WB / 2005 / Real-time PCR for quantitation of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in naturally-infected and challenged pig
Fenaux M / 2002 / Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: Characterization of clinical d
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