지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.9
- 수록면
- 231 - 238 (8page)
이용수
초록· 키워드
오류제보하기
Evaluation of potentials of chemicals to alter expression of genes that are involved in carcinogenesis may serve useful tools in toxicological research. In this investigation, we developed reporter cell lines that expressed luciferase in response to transactivation of hypoxia inducible factor-1, P53 tumor suppressor and Nur77 of which roles have been well established in cancer development and progression. Whereas these reporter cell lines displayed low constitutive backgrounds, the reporter activities were significantly enhanced in response to desferriosamine/CoCl₂, adriamycin or 6-mercaptopurine, which are hypoxia mimicking chemicals, P53 activator or Nur77 inducer, respectively. The activation of the reporter was time- and dose-dependent. Known tumor initiators and promoters, such as phorbol 12-myristate 13-acetate and phorbol 12,13-dicaprinate induced the reporter activity at as low as 10 nM in these stable cell lines. Further, known anti-tumor promoters, such as ascorbic acid and β-carotene repressed the reporter activities. These results indicate that our stable reporter cell lines could serve as a useful system for rapid assessment of carcinogenicity of toxic chemicals.
목차
서론
재료 및 방법
결과 및 고찰
감사의 글
참고문헌
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Cytochrome p450 1A와 어류 세포를 이용한 reporter gene 시험법의 개발
환경독성보건학회 심포지엄 및 학술대회
2011 .10
-
A New Reporter Vector System Based on Flow-Cytometry to Detect Promoter Activity
Immune Network
2009 .12
-
Establishment of a Stable Cell Line Expressing Green Fluorescence Protein-fused Hypoxia Inducible Factor-1α for Assessment of Carcinogenicity of Chemical Toxicants
Toxicological Research
2009 .12
-
유전자 발현 영상기법
대한핵의학회지
2000 .01
-
Constructing a dual-fluorescence reporter system to monitor the dynamic progression of pluripotent cell differentiation
한국실험동물학회 학술발표대회 논문집
2016 .02
-
A fish-specific reporter gene system for the assessment of estrogen compounds in surface water
환경독성보건학회 심포지엄 및 학술대회
2006 .11
-
Optical Imaging for Stem Cell Differentiation to Neuronal Lineage
Nuclear medicine and molecular imaging : NMMI
2012 .01
-
MEASUREMENTS OF ESTROGEN LIKE AND DIOXIN LIKE ACTIVITIES IN KOREAN RIVER WATER USING REPORTER GENE SYSTEM
대한약학회 학술대회
2000 .01
-
Luciferase reporter gene assay를 이용하는 丹蔘추출물의 소염 및 진통작용에 대한 in vitro 연구
동의생리병리학회지
2004 .06
-
핵의학적 리포터 유전자 영상
대한핵의학회지
2004 .01
-
Pre-validation of new reporter gene assay system to detect dioxin-like activity in aquatic environment
환경독성보건학회 심포지엄 및 학술대회
2010 .11
-
Luciferase reporter gene assay를 이용한 丹蔘추출물의 소염, 진통작용에 대한 in vitro 연구
대한한의학회지
2008 .07
-
Molecular imaging of atherosclerosis using reporter gene system
대한방사성의약품학회지
2018 .01
-
Promising Applicability of Reporter Gene Transgenic Rodents in the Toxicological Field
한국실험동물학회 학술발표대회 논문집
2009 .08
-
Hypoxia Induced Multidrug Resistance of Laryngeal Cancer Cells via Hypoxia-inducible Factor-1α
Asian Pacific journal of cancer prevention : APJCP
2013 .01
-
Expressions of a Mouse Neurotrimin Promoter-driven GFP/LacZ Dual Reporter in Differentiating Neural Cell Lineages
대한생식의학회 학술대회
2006 .01
-
Development of Inhibitors Targeting Hypoxia-Inducible Factor 1 and 2 for Cancer Therapy
Yonsei Medical Journal
2017 .01
-
Production of mutated porcine embryos using zinc finger nucleases and a reporter-based cell enrichment system
한국실험동물학회 학술발표대회 논문집
2013 .08
-
The Effect of HIF-1α siRNA on Growth and Chemosensitivity of Mia-paca Cell Line
Yonsei Medical Journal
2008 .01
-
Downstream Reporter Gene Imaging for Signal Transduction Pathway of Dopamine Type 2 Receptor
대한핵의학회 학술대회
2004 .01
이 논문의 저자 정보
이 논문과 함께 이용한 논문
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2009-513-016245980