지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.48 No.4
- 발행연도
- 2007.8
- 수록면
- 217 - 223 (7page)
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초록· 키워드
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The cellular localization of DcHSP17.7, a small heat shock protein (smHSP) from carrot (Daucus carota L.), was studied using green fluorescent protein (GFP) as a marker. A fusion gene construct, the DcHSP17.7 gene fused with the GFP gene, driven by the cauliflower mosaic virus 35S promoter was introduced into potato (Solanum tuberosum L. cv. ‘Desiree’) via Agrobacterium-mediated transformation. Transgenic plants were successfully regenerated and the DcHSP17.7-GFP fusion protein was observed using confocal laser scanning microscopy. Our results showed that DcHSP17.7 is localized in the cytoplasm and nucleus of the cells in leaf and stem tissues. Its general cellular compartmentation did not alter after heat stress (40℃, 4 hours). However, DcHSP17.7 was present at a higher level in the perinuclear region of the cytoplasm than inside the nucleus in the heat-stressed cells of stem tissues. The possible roles of DcHSPI7.7 in enhancement of thermotolerance under heat stress conditions were discussed based on its cellular localization. The integration of the transgene into the genome of transgenic plants and the accumulation of the fusion protein were confirmed by using polymerase chain reaction and immunodetection analyses, respectively.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Literature Cited
참고문헌 (33)
참고문헌 신청
1. [학술지(정기간행물)] - Ahn, Y.-J / 2004 / Genotypic differences in the heat shock response and thermotolerance in four potato cultivars 166 : 901 ~ 911
2. [학술지(정기간행물)] - Ahn, Y.-J / 2006 / Introduction of the carrot HSP17.7 into potato (Solanum tuberosum L.) enhances cellular membrane stability and tuberization in vitro. 29 : 95 ~ 104
3. [학술지(정기간행물)] - Arrigo / / A.P. and W.J. Welch. 1987. Characterization and purification of the small 28 : 15359 ~ 15369
4. [학술지(정기간행물)] - Arrigo / / and W.J. Welch. 1988. Dynamic changes in the structure and intracellular locale of the mammalian low-molecular-weight heat shock protein. Mol. Cell. Biol. 8 : 5059 ~ 5071
5. [학술지(정기간행물)] - Bradford / / M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72 : 248 ~ 254
2. [학술지(정기간행물)] - Ahn, Y.-J / 2006 / Introduction of the carrot HSP17.7 into potato (Solanum tuberosum L.) enhances cellular membrane stability and tuberization in vitro. 29 : 95 ~ 104
3. [학술지(정기간행물)] - Arrigo / / A.P. and W.J. Welch. 1987. Characterization and purification of the small 28 : 15359 ~ 15369
4. [학술지(정기간행물)] - Arrigo / / and W.J. Welch. 1988. Dynamic changes in the structure and intracellular locale of the mammalian low-molecular-weight heat shock protein. Mol. Cell. Biol. 8 : 5059 ~ 5071
5. [학술지(정기간행물)] - Bradford / / M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72 : 248 ~ 254
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UCI(KEPA) : I410-ECN-0101-2009-525-017027199