지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Plant transformation systems have been developed using several different approaches, and the development of reliable and efficient transformation systems is still one of the most important breakthroughs in molecular biology and biotechnology of rice. However, there are many cultivars in rice and transformation efficiency is dependent on the cultivar. The conventional Agrobacterium-mediated rice transformation system using secondary calli requires 14-16 weeks for establishing transgenic plantlets,and it is thought that somaclonal variation through the Tos17 retrotransposon occurs during the tissue culture stage. Here, we modified an Agrobacterium-mediated transformation system in rice cultivar Dongjin, adding an endosperm removal step that is critical to transformation frequency and changing the Agrobacterium strain and media composition. This method increased transformation efficiency and stability. These results will provide valuable information for scientists to use a reliable and efficient rice transformation system.
목차
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참고문헌 (19)
참고문헌 신청
Chan MT / 1993 / Agrobacterium-mediated production of transgenic rice plants expressing a chimeric alpha-amylase promoter/beta-glucuronidase gene / Plant Mol Biol 22 : 491 ~
Chilton MD / 1974 / Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors / Proc Natl Acad Sci U S A 71 : 3672 ~ 3676
Christou P / 1995 / Particle bombardment / Methods Cell Biol 50 : 375 ~ 382
Gelvin SB / 2003 / Agrobacterium-mediated plant transformation: the biology behind the “gene-jockeying” tool / Microbiol Mol Biol Rev 67 : 16 ~ 37
Hayashimoto A / 1990 / A polyethylene glycol-mediated protoplast transformation system for production of fertile transgenic rice plants / Plant Physiol 93 : 857 ~ 863
Chilton MD / 1974 / Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors / Proc Natl Acad Sci U S A 71 : 3672 ~ 3676
Christou P / 1995 / Particle bombardment / Methods Cell Biol 50 : 375 ~ 382
Gelvin SB / 2003 / Agrobacterium-mediated plant transformation: the biology behind the “gene-jockeying” tool / Microbiol Mol Biol Rev 67 : 16 ~ 37
Hayashimoto A / 1990 / A polyethylene glycol-mediated protoplast transformation system for production of fertile transgenic rice plants / Plant Physiol 93 : 857 ~ 863
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