지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.51 No.3
- 발행연도
- 2010.6
- 수록면
- 141 - 145 (5page)
이용수
초록· 키워드
오류제보하기
DcHsp17.7 is a heat-inducible small heat shock protein (sHSP) in carrot (Daucus carota L.). Moreover, here we report that DcHsp17.7 is constitutively expressed in vegetative and reproductive tissues at defined developmental stages. Immunoblot analysis using a polyclonal antibody raised against DcHsp17.7 showed that the protein was present in non-stressed leaf tissue and gradually accumulated upon heat stress (40℃). In the heat-induced level of DcHsp17.7, there were transient, but reproducible declines, suggesting dynamic expression regulation. The protein was also present in dry and germinating seeds. It is worth noting that unlike most other seed sHSPs, which disappear upon completion of germination, DcHsp17.7 was continuously present in young seedling. In flower bud and open flower, the protein was constitutively expressed. Heat stress increased the amount of DcHsp17.7 in these floral tissues. Under the opposite temperature extreme, cold stress (2℃), DcHsp17.7 was also present in these tissues, but the levels were lower than those under heat stress. Constitutive expression of DcHsp17.7 suggests that it may play physiological role(s) in the absence of thermal stress. Alternatively, DcHsp17.7 in non-stressed tissues may confer advanced protection to sudden temperature changes by immediately enhancing thermotolerance of cells.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Literature Cited
참고문헌 (22)
참고문헌 신청
1. [학술지(정기간행물)] - Ahn, Y-J. / 2006 / Introduction of the carrot HSP17.7 into potato (Solanum tuberosum L.) enhances cellular membrane stability and tuberization in vitro / Plant Cell Environ 29 : 95 ~ 104
2. [학술지(정기간행물)] - Ahn, Y-J. / 2004 / Genotypic differences in the heat-shock response and thermotolerance in four potato cultivars / Plant Sci 166 : 901 ~ 911
3. [학술지(정기간행물)] - Almoguera, C. / 1992 / Developmental and environmental concurrent expression of sunflower dry-seed-stored low-molecular-weight heat-shock protein and Lea mRNAs / Plant Mol. Biol 19 : 781 ~ 792
4. [학술지(정기간행물)] - Bradford, M.M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding / Anal. Biochem 72 : 248 ~ 254
5. [학술지(정기간행물)] - Carranco, R. / 1997 / A plant small heat shock protein gene expressed during zygotic embryogenesis but noninducible by heat stress / J. Biol. Chem 272 : 27470 ~ 27475
2. [학술지(정기간행물)] - Ahn, Y-J. / 2004 / Genotypic differences in the heat-shock response and thermotolerance in four potato cultivars / Plant Sci 166 : 901 ~ 911
3. [학술지(정기간행물)] - Almoguera, C. / 1992 / Developmental and environmental concurrent expression of sunflower dry-seed-stored low-molecular-weight heat-shock protein and Lea mRNAs / Plant Mol. Biol 19 : 781 ~ 792
4. [학술지(정기간행물)] - Bradford, M.M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding / Anal. Biochem 72 : 248 ~ 254
5. [학술지(정기간행물)] - Carranco, R. / 1997 / A plant small heat shock protein gene expressed during zygotic embryogenesis but noninducible by heat stress / J. Biol. Chem 272 : 27470 ~ 27475
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UCI(KEPA) : I410-ECN-0101-2010-525-002463339