지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Macrophage migration inhibitory factor (MIF), known as a cytokine, is a multifunctional protein that is ubiquitously expressed in a variety of cells and tissues; however, enzymatic function of MIF still remains elusive in cells. In this study, we assessed details of the tautomerase activity of MIF. We established rapid purification condition for MIF by using pGEX system and compared the L-dopachrome tautomerase activity of GST-MIF, tMIF, and MIF. The results show that GST (glutathione S-transferase)-epitope tag or N-terminal amino acids flanking the essential P²almost completely abrogated L-dopachrome tautomerase activity of MIF. Subsequently, to determine whether the N-terminal tags have effects on oligomerization of MIF, protein cross-linking products were analyzed on 15% SDS-PAGE. The result demonstrates that N-terminal tags are dispensable for the formation of MIF's homooligomers. Thus, the results imply that exposure of P²containing hydrophobic pocket in the active site is critical for L-dopachrome tautomerase activity of MIF. In addition, our study suggest that the MIF's tautomerase activity might be influenced by interacting with cellular partners.
목차
Abstract
재료 및 방법
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감사의 글
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참고문헌 (23)
참고문헌 신청
Al-Abed, Y., D. Dabideen, B. Aljabari, A. Valster, D. Messmer, M. Ochani, M. Tanovic, K. Ochani, M. Bacher, F. Nicoletti, C. N. Metz, V. A. Pavlov, E. J. Miller and K. J. Tracey. 2005. ISO-1 binding to the tautomerase active site of MIF inhibits its pro-i
Baugh, J. A. and R. Bucala. 2002. Macrophage migration inhibitory factor. Crit Care Med 30, S27-S35.
Baumann, R., C. Casaulta, D. Simon, S. Conus, S. Yousefi and H. U. Simon. 2003. Macrophage migration inhibitory factor delays apoptosis in neutrophils by inhibiting the mitochondria-dependent death pathway. Faseb J 17, 2221-2230.
Bendrat, K., Y. Al-Abed, D. J. Callaway, T. Peng, T. Calandra, C. N. Metz and R. Bucala. 1997. Biochemical and mutational investigations of the enzymatic activity of macrophage migration inhibitory factor. Biochemistry 36, 15356-15362.
Bernhagen, J., R. A. Mitchell, T. Calandra, W. Voelter, A. Cerami and R. Bucala. 1994. Purification, bioactivity, and secondary structure analysis of mouse and human macrophage migration inhibitory factor (MIF). Biochemistry 33, 14144-14155.
Baugh, J. A. and R. Bucala. 2002. Macrophage migration inhibitory factor. Crit Care Med 30, S27-S35.
Baumann, R., C. Casaulta, D. Simon, S. Conus, S. Yousefi and H. U. Simon. 2003. Macrophage migration inhibitory factor delays apoptosis in neutrophils by inhibiting the mitochondria-dependent death pathway. Faseb J 17, 2221-2230.
Bendrat, K., Y. Al-Abed, D. J. Callaway, T. Peng, T. Calandra, C. N. Metz and R. Bucala. 1997. Biochemical and mutational investigations of the enzymatic activity of macrophage migration inhibitory factor. Biochemistry 36, 15356-15362.
Bernhagen, J., R. A. Mitchell, T. Calandra, W. Voelter, A. Cerami and R. Bucala. 1994. Purification, bioactivity, and secondary structure analysis of mouse and human macrophage migration inhibitory factor (MIF). Biochemistry 33, 14144-14155.
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UCI(KEPA) : I410-ECN-0101-2009-470-015375981