지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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We investigated microRNA (miRNA) biogenesis of Epstein-Barr virus (EBV) which is the first virus shown to produce viral miRNAs. As expected, expression of all the reported EBV miRNAs were detected by Northen blot in an EBV-infected B cell line, B95-8; BHRF1-1, BHRF1-2, BHRF1-3, BART1, and BART2. The putative EBV pri-miRNAs and pre-miRNAs predicted from the known mature EBV miRNA sequences were detected by RT-PCR in B95-8 cells. Many animal miRNA genes exist as clusters of 2-7 genes and they are expressed polycistronically. As the EBV miRNAs are clustered in two regions of the EBV genome, we examined whether these clustered EBV miRNA genes are also expressed polycistronically. A long polycistronic transcript with the expected size (1602 bp) corresponding to the BHRF1-1～BHRF1-2～BHRF1-3 was amplified. However, any polycistronic transcript containing both BART1 and BART2 was detectable in B95-8. These results suggest that EBV miRNAs may be processed in a similar way with animal miRNAs and that some of the clustered EBV miRNAs can be transcribed polycistronically.
목차
Abstract
재료 및 방법
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감사의 글
참고문헌
참고문헌 (33)
참고문헌 신청
Aman, P., B. Ehlin-Henriksson and G. Klein. 1984. Epstein-Barr virus susceptibility of normal human B lymphocyte populations. J. Exp. Med. 159, 208-220.
Ambros, V., B. Bartel, D. P. Bartel, C. B. Burge, J. C. Carrington, X. Chen, G. Dreyfuss, S. R. Eddy, S. Griffiths-Jones, M. Marshall, M. Matzke, G. Ruvkun and T. Tuschl. 2003. A uniform system for microRNA annotation. RNA 9, 277-279.
Bartel, D. P. 2004. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116, 281-297.
Bernstein, E., A. A. Caudy, S. M. Hammond and G. J. Hannon. 2001. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409, 363-366.
Bohnsack, M. T., K. Czaplinski and D. Gorlich. 2004. Exportin 5 is a RanGTP-dependent dsRNA-binding protein that mediates nuclear export of pre-miRNAs. RNA 10, 185-191.
Ambros, V., B. Bartel, D. P. Bartel, C. B. Burge, J. C. Carrington, X. Chen, G. Dreyfuss, S. R. Eddy, S. Griffiths-Jones, M. Marshall, M. Matzke, G. Ruvkun and T. Tuschl. 2003. A uniform system for microRNA annotation. RNA 9, 277-279.
Bartel, D. P. 2004. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116, 281-297.
Bernstein, E., A. A. Caudy, S. M. Hammond and G. J. Hannon. 2001. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409, 363-366.
Bohnsack, M. T., K. Czaplinski and D. Gorlich. 2004. Exportin 5 is a RanGTP-dependent dsRNA-binding protein that mediates nuclear export of pre-miRNAs. RNA 10, 185-191.
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UCI(KEPA) : I410-ECN-0101-2009-470-015375901