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자료유형
학술저널
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한국식품영양과학회 Journal of Food Science and Nutrition Journal of Food Science and Nutrition Vol.4 No.3
발행연도
1999.9
수록면
203 - 208 (6page)

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The effect of the polyunsaturated fatty acids, linoleic acid (LA), arachidonic acid (AA) and conjugated dienoic linoleic acid (CLA) on IEC-6 cells (rat intestinal cell) proliferation and cell transduction have been determined in vitro. IEC-6 cells proliferation was assessed by cell growth and [³H]-thymidine incorporation analysis. At 10 μM concentration, the proliferation of cells supplemented with AA or LA was significantly higher than that of CLA. [³H]-thymidine uptake showed the same results. LA and AA increased [³H]-thymidine uptake more than CLA. The stimulatory effect of LA or AA was even more pronounced in the presence of IGF. Both cell number analysis and [³H]-thymidine incorporation revealed that IEC-6 cell proliferation was influenced differently by exogenous free fatty acids, in which AA or LA stimulated IEC-6 cell proliferation and CLA inhibited it. Tyrosine phosphorylation provides a key switch to regulate cellular activity in response to extracellular stimuli. At 20 μM and 10 μM, AA with IGF-1 stimulated protein tyrosine phosphorylation in IEC-6 cells, but LA’s impact was less than that of AA. CLA and CLA with IGF-1 inhibited protein tyrosine phosphorylation in IEC-6 cells. These results suggest there is a possible correlation between cell proliferation and IGF receptor tyrosine kinase activity driven by AA.

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Abstract

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

ACKNOWLEDGEMENTS

REFERENCES

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