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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2002.9
- 수록면
- 227 - 232 (6page)
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초록· 키워드
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In order to find out the appropriate in vitro method for high correlation with in vivo, we compared the sensitivities of phototoxicity (PT) in vitro method between in human keratinocytes, HaCaT cells and in 3T3 fibroblast cells derived from Balb/c mice. Both cells were exposed to six known phototoxic chemicals : promethazine, neutral red, chlortetracycline, amiodarone, bithional, 8-methoxypsoralen, or non-phototoxic chemical, ALS (ammonium laureth sulfate) and then irradiated with 5 J/㎠ of UVA. Cell viability (IC_(50)) was measured by neutral red uptake (NRU) assay. The ratio of IC_(50) value of chemicals in the presence and absence of UVA was determined by the cut-off value. The phototoxic potential of test chemicals in NRU assay was determined by measuring the photoirritation factor (PIF) with a cut-off value of 5. In both 3T3 and HaCaT cells, all known phototoxic chemicals were positive (over 5 of PIF value), except that bithional was found to be non-phototoxic to HaCaT cells, and ALS, non-phototoxic chemical was negative. These results suggest that Balb/c 3T3 cell was more sensitive than HaCaT cell to predict phototoxicity potential.
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UCI(KEPA) : I410-ECN-0101-2009-513-013456454