지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한치과마취과학회 Journal of dental anesthesia and pain medicine Journal of dental anesthesia and pain medicine 제14권 제1호
- 발행연도
- 2014.1
- 수록면
- 41 - 47 (7page)
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초록· 키워드
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Background: Autophagy is a self-eating process that is important for balancing sources of energy at critical times in development and in response stress. Autophagy also plays a protective role in removing clearing damaged intracellular organelles and aggregated proteins as well as eliminating intracellular pathogens. The purpose of the present study was to examine the protective effect of propofol against hypoxic damage using keratinocytes. Methods: Human keratinocytes (HaCaT cells) were obtained from the American Type Culture Collection. Propofol which were made by dissolving them in DMSO were kept frozen at $-4^{\circ}C$ until use. The stock was diluted to their concentration with DMEM when needed. Prior to propofol treatment cells were grown to about 80% confluence and then exposed to propofol at different concentrations (0, 25, 50, 75, $100{\mu}M$) for 2 h pretreatment. Cell viability was measured using a quantitative colorimetric assay with thiazolyl blue tetrazolium bromide (MTT assay), and fluorescence microscopy and western blot analysis were used for evaluation of autophagy processes. Results: The viability of propofol-treated HaCaT cells was increased in a dose-dependent manner. Propofol did not show any significant toxic effect on the HaCaT cells. The autophagy inhibitor, 3-methyladenine, reduced cell viability of hypoxia-injured HaCat cells. Fluorescence microscopy and western blot analysis showed propofol induce autophagy pathway signals. Conclusions: Propofol enhanced viability of hypoxia-injured HaCaT cells and we suggest propofol has cellular protective effects by autophagy signal pathway activation.
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참고문헌 (17)
참고문헌 신청
Martin P / 1997 / Wound healing--aiming for perfect skin regeneration / Science 276(5309):75~81
Buisson AC / 1996 / Wound repair-induced expression of a stromelysins is associated with the acquisition of a mesenchymal phenotype in human respiratory epithelial cells / Lab Invest 74(3):658~669
Laderoute KR / 1997 / Hypoxia/reoxygenation stimulates Jun kinase activity through redox signaling in cardiac myocytes / Circ Res 80(3):336~344
Nakatogawa H / 2009 / Dynamics and diversity in autophagy mechanisms: lessons from yeast / Nat Rev Mol Cell Biol 10(7):458~467
Levine B / 2008 / Autophagy in the pathogenesis of disease / Cell 132(1):27~42
Buisson AC / 1996 / Wound repair-induced expression of a stromelysins is associated with the acquisition of a mesenchymal phenotype in human respiratory epithelial cells / Lab Invest 74(3):658~669
Laderoute KR / 1997 / Hypoxia/reoxygenation stimulates Jun kinase activity through redox signaling in cardiac myocytes / Circ Res 80(3):336~344
Nakatogawa H / 2009 / Dynamics and diversity in autophagy mechanisms: lessons from yeast / Nat Rev Mol Cell Biol 10(7):458~467
Levine B / 2008 / Autophagy in the pathogenesis of disease / Cell 132(1):27~42
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