Intense pulsed light (IPL) sterilization is a novel technology that can extend the shelf-life of fresh food, it can be sterilized products without heat generation in the products. This study examine the sterilization effects of IPL treatment depending on type of treatment chamber materials and packaging film. According to the measurement of the light transmission rate of the treatment chamber materials in pulsed light treatment, there were large differences in the transmission rate of UV-C depending on the materials as the plate made of 1 mm thick quartz, acryl and glass had 74%, 35% and 1% transmission rate, respectively against the control. When using the plate made of 1mm thick quartz, 2.8 log of bacteria were killed after 10 seconds treatment, and all bacteria were killed after 60 seconds treatment. The sterilization rate did not changed much when the thickness of the material changed to 2-3 mm. When using acryl, after 180 seconds treatment, 5 log of bacteria were killed in 1mm thickness, 0.5 log of bacteria were killed in 2 mm thickness and 1 log of bacteria were killed in 3 mm thickness. This showed that the thickness of acryl had a huge impact on the sterilization rate of bacteria. In IPL treatment, the sterilization rate of E. coli was the highest in the plate made of quartz, followed by those made of acryl and glass. In addition, it was identified that there were significant impacts of the materials and thickness of plate on eradication of bacteria since the transmission rate declined as the thickness of quartz, acryl and glass increased. The study identified the sterilization effects of pulsed light depending on the packaging film materials including PP, OPP, PE and PET that are commonly used as packaging materials in the market for fresh foods. According to the measurement of the light transmission rate of each packaging films material in pulsed light treatment, there were differences in the UV-C transmission rate depending on the materials as the rate of PP, OPP, PE and PET was 91.3%, 89.7%, 89.5% and 1.8%, respectively. In terms of the packaging film made of 30 thick PP, around 6 log of bacteria were killed after 20 seconds treatment, and all bacteria were killed after 60 seconds treatment. There were no big differences in the sterilization rate depending on thickness, but the rate tended to be gradually reduced. In terms of the containers made of 30 thick OPP and PE, 6 log of bacteria were killed after 30 seconds treatment, and all bacteria were killed after 60 seconds treatment. PET had lower sterilization effects than other packaging materials. In terms of the light transmission rate by film material, there were not difference between the materials, except for PET, but PP had the highest transmission rate among them. As the packaging film materials thickened, the light transmission rate tended to be decreased, but did not have significant differences, except for PET. This study found that quartz has the highest sterilization effect among the materials of treatment chamber in IPL treatment, and PP is the best material for food packaging film. It is necessary to continue to carry out researches on effective materials of treatment chamber and packaging film to industrialize the IPL treatment system.