지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 洪承旭
- 발행연도
- 2017
- 저작권
- 동국대학교 논문은 저작권에 의해 보호받습니다.
이용수3
이 논문의 연구 히스토리 (2)
- 이 논문의 후속연구가 궁금하신가요?
- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
- 이 논문의 연구 히스토리 확인하기
초록· 키워드
오류제보하기
Objectives
Atopic dermatitis is a skin disease caused by immunological, genetic and environmental factors. It accompanies with severe pruritus and collapse of skin barrier, inflammation, increases of IgE in serum, infiltration of neutrophil and Th2 hyperplasia at inflammatory site.
Maifanite contains silicate mainly and could be used as an ointment for skin disease. However, there have been few studies about maifanite uses for atopic dermatitis. In this regards, we report the anti-inflammatory and promoting skin recovery effects of ionized maifanite on damaged skin barrier with experimentally elicited atopic dermatitis using D. pteronyssinus antigen of NC/Nga mice.
Methods
Nc/Nga mice of gestational age 8 week were chosen and divided into 3 groups: 1) control group(CON), 2) atopic dermatitis elicited group(AE group), 3) ionized maifanite treated group after atopic dermatitis elicitation(MT group).
5% SDS was applied for damaging skin barrier. And D. pteronyssinus crude extract also applied for 3 weeks and elicited skin disease which resembles atopic dermatitis. MT group was treated for 3 weeks with ionized maifanite. Ionized maifanite was applied once a day and voluntarily administrated. AE group and control group were treated with normal saline in the same way. Skin damages, p-ERK1/2, MMP-9, substance P, angiogenesis, IL-4, STAT-6, NF-κB, iNOS changes were observed on atopic dermatitis elicited region.
Results
In MT group, skin lesions like eczema were more improved than AE group. p-ERK1/2 positive reaction was stronger in AE group than in control group, and reduced in MT group. MMP-9 and substance P positive reaction at dermal papillae was also stronger in AE group than in CON group, and reduced in MT group. With skin angiogram, capillary vessel increased more in AE group than in CON group, and decreased in MT group. Also, IL-4 positive reaction cell and STAT-6 positive reaction cell reduced more in MT group than in AE group. NF-κB p65 positive reaction cell and iNOS positive reaction cell also declined more in MT group than in AE group.
Conclusion
It is supposed that ionized maifanite has anti-inflammatory effects on NC/Nga mice’s atopic dermatitis with suppressing IL-4 production and Th2 cell differentiation, and controlling NF-κB activation.
Atopic dermatitis is a skin disease caused by immunological, genetic and environmental factors. It accompanies with severe pruritus and collapse of skin barrier, inflammation, increases of IgE in serum, infiltration of neutrophil and Th2 hyperplasia at inflammatory site.
Maifanite contains silicate mainly and could be used as an ointment for skin disease. However, there have been few studies about maifanite uses for atopic dermatitis. In this regards, we report the anti-inflammatory and promoting skin recovery effects of ionized maifanite on damaged skin barrier with experimentally elicited atopic dermatitis using D. pteronyssinus antigen of NC/Nga mice.
Methods
Nc/Nga mice of gestational age 8 week were chosen and divided into 3 groups: 1) control group(CON), 2) atopic dermatitis elicited group(AE group), 3) ionized maifanite treated group after atopic dermatitis elicitation(MT group).
5% SDS was applied for damaging skin barrier. And D. pteronyssinus crude extract also applied for 3 weeks and elicited skin disease which resembles atopic dermatitis. MT group was treated for 3 weeks with ionized maifanite. Ionized maifanite was applied once a day and voluntarily administrated. AE group and control group were treated with normal saline in the same way. Skin damages, p-ERK1/2, MMP-9, substance P, angiogenesis, IL-4, STAT-6, NF-κB, iNOS changes were observed on atopic dermatitis elicited region.
Results
In MT group, skin lesions like eczema were more improved than AE group. p-ERK1/2 positive reaction was stronger in AE group than in control group, and reduced in MT group. MMP-9 and substance P positive reaction at dermal papillae was also stronger in AE group than in CON group, and reduced in MT group. With skin angiogram, capillary vessel increased more in AE group than in CON group, and decreased in MT group. Also, IL-4 positive reaction cell and STAT-6 positive reaction cell reduced more in MT group than in AE group. NF-κB p65 positive reaction cell and iNOS positive reaction cell also declined more in MT group than in AE group.
Conclusion
It is supposed that ionized maifanite has anti-inflammatory effects on NC/Nga mice’s atopic dermatitis with suppressing IL-4 production and Th2 cell differentiation, and controlling NF-κB activation.
목차
Ⅰ. 서론 1Ⅱ. 재료 및 방법 31. 실험 동물 32. 아토피 피부염의 유발 33. 이온화 된 맥반석의 제조 및 투여 34. 피부 조직 표본 제작 45. 피부 손상에 대한 효과 관찰 41) 상피 세포 과형성 변화 42) 진피 내 아교섬유 분포 변화 53) 아교섬유분해효소 변화 54) 비만세포 변화 55) 진피 내 신경펩티드 변화 66. 혈관 신생성 억제 효과 관찰 61) 혈관 신생성의 영상분석 변화 62) 진피 유두 내 혈관 분포 변화 67. IL-4 생성 억제 효과 관찰 68. NF-κB 조절을 통한 항염증 효과 관찰 79. 영상분석과 통계처리 7Ⅲ. 결과 81. 피부 손상에 대한 효과 81) 외부 피부 형태 및 조직 손상 완화 82) 상피 세포 과형성 조절을 통한 세포 증식 억제 103) 진피 내 아교 섬유 분포 변화 124) 비만세포 분포 변화 145) 진피 내 신경펩티드 변화 142. 혈관 신생성 억제 효과 163. IL-4 생성 억제 효과 194. NF-κB 활성 억제 효과 21Ⅳ. 고찰 24Ⅴ. 결론 31Ⅵ. 참고문헌 32ABSTRACT 40
최근 본 자료
댓글(0)
0