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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 박용진
- 발행연도
- 2017
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Objective of this study was to investigate the difference of cadmium (Cd) levels in rice grains from non-polluted fields and to define the gene associated with Cd uptake for producing safety food. Cd was analyzed by Inductively Coupled Plasma Mass Spectrometer (ICP-MS). The average concentration of Cd in rice grains was 0.943 ㎍/㎏ and Cd levels ranged from 0.050 to 5.699 ㎍/㎏.
Genome-Wide Association study (GWAS) based on phenotype data for Cd levels was performed. However, results of GWAS were affected by subpopulation structure and caused false positive. Therefore, GWAS for rice ecotypes (temperate Japonica, tropical Japonica, Indica, Aus, Aromatic, and Admixture) was performed to minimize false positive. GWAS results showed that Os01g0611300, Os01g0611900, Os01g0611950, Os01g0612000, Os01g0612200, Os11g0444400, Os11g0444700, Os11g0444800, and Os11g0444900 genes have significant correlation with Cd levels in rice grains. The sequences of these genes were compared to sequence positions of each other gene (haplotype analysis). According to the results of haplotype analysis, Cd levels of non-synonymous group were higher than other groups and sequence of non-synonymous group was similar to that of Indica. These results were corresponding to the previous research result that Cd levels of Indica were higher than Japonica. Therefore, candidate genes detected through GWAS need to be examined by knock-out or cross breeding.
Genome-Wide Association study (GWAS) based on phenotype data for Cd levels was performed. However, results of GWAS were affected by subpopulation structure and caused false positive. Therefore, GWAS for rice ecotypes (temperate Japonica, tropical Japonica, Indica, Aus, Aromatic, and Admixture) was performed to minimize false positive. GWAS results showed that Os01g0611300, Os01g0611900, Os01g0611950, Os01g0612000, Os01g0612200, Os11g0444400, Os11g0444700, Os11g0444800, and Os11g0444900 genes have significant correlation with Cd levels in rice grains. The sequences of these genes were compared to sequence positions of each other gene (haplotype analysis). According to the results of haplotype analysis, Cd levels of non-synonymous group were higher than other groups and sequence of non-synonymous group was similar to that of Indica. These results were corresponding to the previous research result that Cd levels of Indica were higher than Japonica. Therefore, candidate genes detected through GWAS need to be examined by knock-out or cross breeding.
목차
목 차Ⅰ. 서 론 1Ⅱ. 연 구 사 31. 벼 재배 현황 및 연구의 중요성 32. 벼 종자 내 카드뮴 연구 동향 43. 벼 핵심집단의 중요성 54. GWAS(Genome-Wide Association Study) 연구 동향 5Ⅲ. 재료 및 방법 71. 벼 공시재료 72. DNA추출 및 유전체 재분석 73. 벼 등숙기 종자 내 카드뮴 함량 분석 84. Variant calling 85. 전장유전체연관분석(Genome-Wide Association Study) 9Ⅳ. 결 과 101. 벼 등숙기 종자 내 카드뮴 함량분석 결과 112. 벼 유전체 재분석 113. 유전체 재분석 자원의 전장유전체연관분석(GWAS) 및 후보유전자 확인 134. 온대자포니카 품종의 전장유전체연관분석(GWAS) 및 후보유전자 확인 205. 후보유전자들의 haplotype 분석 및 아미노산 조성 변화 22Ⅴ. 종합 고찰 37Ⅵ. 적 요 39참고 문헌 40ABSTRACT 44Appendix 46
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