As Allium hookeri (A. hookeri) is known as a healthy food containing a larger amount of sulfur compounds than commonly known alliaceous plants, the number of farms growing it and its consumption have been increasing. Consequently, research on the antioxidant, anti-inflammatory, and anti-cancer effects of A. hookeri is being conducted actively; however, research on its clinical applications is lacking. Accordingly, in this study, after preparing water and ethanol extracts of A. hookeri roots, the antioxidant and anti-inflammatory effects were compared between the two types of extracts. The more effective extracts were then orally administered to ICR mice for a week and the degree of DNA damage was determined; the effect of A. hookeri was determined by comparing the normal group, the control group to which alcohol was administered, and the treatment groups with 0.25%, 0.5%, and 1% A. hookeri root ethanol extracts, by inducing acute alcohol hepatotoxicity with 40% alcohol, and exploring blood alcohol levels, change in AST and ALT, and activity of ADH and ALDH. A. hookeri root 95% ethanol extracts revealed antioxidant activity(total polyphenol and DPPH and ABTS radical scavenging activity, FRAP value) that was superior to that of water extracts(p<0.05). Raw 264.7 cells were treated with 31.25 ~ 1,000 μg/mL concentrations of water extracts and 95% ethanol extracts, separately. According to the results, cell toxicity was observed in the groups with 1,000 μg/mL of water extract treatment, while 95% ethnoal extracts showed cell viability of over 100% in all of the concentrations. In the case of concentrations without cell toxicity (less than 1,000 μg/mL) water extracts restricted nitric oxide generation by about 3% ~ 44% compared to the control group, while 95% ethanol extracts (31.25 ~ 1,000 μg/mL) restricted 6 ~ 55%. The results showed the superior effects of 95% ethanol extracts to water extracts, as production of pro-inflammatory cytokines TNF-α showed a dose-dependent reduction effect of up to 250 ? 1,000 μg/mL, and 95% ethanol extracts showed a dose-dependent reduction effect of up to 125 ? 1,000 μg/mL. According to the results on the effects of A. hookeri root ethanol extracts on DNA damage observed by an oral administration of 0.25%, 0.5%, and 1% A. hookeri root ethanol extract in comparison to control administered with distilled water to 8 week-old male ICR mice for a week, the groups with the higher dose and moderate dose administration of A. hookeri root ethanol extracts showed significant lower damage (p<0.05). The mouses blood alcohol concentration showed that treatment group showed significant differences compared to controls (p<0.05). Serum AST measurements showed that the group administered with 0.25% A. hookeri root ethanol extracts did not show significant difference from controls; however, the groups with 0.5% and 1% administration did (p<0.05), and the group with a high-dose administration showed a decrease to the same level as the normal group. The results on serum ALT activity showed that treatment groups showed significant dose-dependent differences compared to controls (p<0.05), and the group with the high-dose administration showed similar activity to the normal group, suggesting little liver damage. The RT-PCR results of ADH and ALDH DNA measured with mRNA of liver tissues showed that, as shown in the low level of blood alcohol concentration, activity was higher in the treatment groups than in the control group. In conclusion, 95% ethanol extracts of A. hookeri root is likely to promote alcohol decomposition and decrease alcohol concentration in the blood and the liver when alcohol is administered by increasing ADH and ALDH activity in the liver, and the results are expected to be used as basic materials for the development of functional food based on A. hookeri root ethanol extracts.