지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 김장현
- 발행연도
- 2015
- 저작권
- 동국대학교 논문은 저작권에 의해 보호받습니다.
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Objectives ; In this study, effect of Geumsuyukgunjeon (GYJ)) on the increase in airway epithelial mucosubstances of rats with acute bronchitis and EGF-induced MUC5AC mucin production from human airway epithelial cells were investigated.
Materials and Methods ; Hypersecretion of airway mucus was induced by exposure of rats to SO2 during 3 weeks. Effect of orally-administered GYJ during 2 weeks on increase in airway epithelial mucosubstances from tracheal goblet cells of rats was assesed using histopathological analysis after staining the epithelial tissue with PAS-alcian blue. Possible cytotoxicity of GYJ was assessed by examining the potential damage of kidney and liver functions by measuring serum GOT/GPT activities and serum BUN and creatinine concentrations of rats and the body weight gain during experiment, after administering GYJ orally. Effect of GYJ on EGF-induced MUC5AC mucin production from human airway epithelial cells (A549) was investigated. Confluent A549 cells were pretreated for 30 min in the presence of GYJ and treated with EGF (25ng/ml) for 24 hrs, to assess the effect of GYJ on EGF-induced MUC5AC mucin production using enzyme-linked immunosorbent assay (ELISA).
Results ;
(1) GYJ decreased the amount of intraepithelial mucosubstances of trachea of rats.
(2) GYJ did not show kidney and liver toxicities and did not affect body weight gain of rats during experiment.
(3) GYJ significantly inhibited EGF-induced MUC5AC mucin production from A549 cells.
Conclusion ; The result from the present study suggests that GYJ might control both the mucus hypersecretion in vivo and do not show in vivo toxicity to liver and kidney functions after oral administration and the production of pulmonary mucin.
Materials and Methods ; Hypersecretion of airway mucus was induced by exposure of rats to SO2 during 3 weeks. Effect of orally-administered GYJ during 2 weeks on increase in airway epithelial mucosubstances from tracheal goblet cells of rats was assesed using histopathological analysis after staining the epithelial tissue with PAS-alcian blue. Possible cytotoxicity of GYJ was assessed by examining the potential damage of kidney and liver functions by measuring serum GOT/GPT activities and serum BUN and creatinine concentrations of rats and the body weight gain during experiment, after administering GYJ orally. Effect of GYJ on EGF-induced MUC5AC mucin production from human airway epithelial cells (A549) was investigated. Confluent A549 cells were pretreated for 30 min in the presence of GYJ and treated with EGF (25ng/ml) for 24 hrs, to assess the effect of GYJ on EGF-induced MUC5AC mucin production using enzyme-linked immunosorbent assay (ELISA).
Results ;
(1) GYJ decreased the amount of intraepithelial mucosubstances of trachea of rats.
(2) GYJ did not show kidney and liver toxicities and did not affect body weight gain of rats during experiment.
(3) GYJ significantly inhibited EGF-induced MUC5AC mucin production from A549 cells.
Conclusion ; The result from the present study suggests that GYJ might control both the mucus hypersecretion in vivo and do not show in vivo toxicity to liver and kidney functions after oral administration and the production of pulmonary mucin.
목차
Ⅰ. 緖 論 1Ⅱ. 材料 및 方法 31. 材料 31) 동물 32) 배양세포 33) 金水六君煎 (GYJ)의 약물 구성 34) GYJ 추출물 분리 45) 시약 42. 方法 51) In vivo 5(1) 기도 점액 과다 분비 흰쥐 모델 작성 5(2) 기도 점액 과다 분비 흰쥐 모델에 GYJ 의 경구투여 6(3) GYJ가 호흡기 배상세포 내 점액 함유량에 미치는 영향 측정 6(4) GYJ의 경구투여가 흰쥐의 신장 기능에 미치는 영향 측정 7(5) GYJ의 경구투여가 흰쥐의 간 기능에 미치는 영향 측정 7(6) GYJ의 경구투여가 흰쥐의 체중 증가에 미치는 영향 측정 82) In vitro 8(1) A549 세포 배양 및 GYJ의 처리 8(2) A549 세포에서의 MUC5AC 뮤신 생성량 측정 93) 통계처리 10Ⅲ. 成 績 111. In vivo 111) 호흡기 상피 배상세포 내의 점액 함유량 증가에 미치는 영향 112) 흰쥐의 신장 기능에 미치는 영향 133) 흰쥐의 간 기능에 미치는 영향 144) 흰쥐의 체중 증가에 미치는 영향 152. In vitro 161) A549 세포에서 EGF로 자극된 MUC5AC 뮤신생성 증가에 미치는 영향 16Ⅳ. 考 察 17Ⅴ. 結 論 23參考文獻 24ABSTRACT 28
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