목차

CHAPTER I. Literature Review 1
1. Introduction 2
2. β-Glucan 3
3. Physiological effects of cereal β-glucans 6
4. Physicochemical characteristics of cereal β-glucans 10
4.1. β-Glucan content 10
4.2. β-Glucan structure 12
4.3. β-Glucan molecular weight 12
4.4. Rheology of β-glucan solutions 13
5. Extraction procedures of cereal β-glucans 15
5.1. Inactivation of endogenous enzymes 15
5.2. Extraction of soluble β-glucan 16
5.3. Extraction of insoluble β-glucan 19
5.4. Precipitation of the β-glucan 20
6. Depolymerization and modification of of cereal β-glucans 21
6.1. Depolymerization 21
6.2. Modification 24
7. Objectives of the study 27
8. References 28
CHAPTER II. Physicochemical characteristics of barley β-glucan with different extraction methods . 48
Abstract 49
I. Introduction 51
II. Materials and methods 55
1. Materials 55
2. Pre-treatment methods 55
3. Extraction of total and soluble β-glucan 57
4. Measurement of β-glucan purity 59
5. Physicochemical characteristics of soluble β-glucan 61
6. Statistical analysis 64
III. Results and discussion 65
1. The content of crude total, soluble and insoluble β-glucan 65
2. The purity of crude total and soluble β-glucan 73
3. The ratio of β-(14)/(13) linkages of soluble β-glucan 81
4. The average molecular weight of soluble β-glucan 86
5. The viscosity of soluble β-glucan 95
6. The resoulbility of soluble β-glucan 103
7. Correlation between extraction methods and physicochemical characteristics 107
IV. References 115
CHAPTER III. In vitro binding capacities of soluble barley β-glucans with different extraction methods. 123
Abstract 124
I. Introduction 126
II. Materials and methods 128
1. Materials 128
2. Pre-treatment methods 128
3. Extraction and purification of soluble barley β-glucan 130
4. In vitro dinging capacity of soluble β-glucan 132
5. Statistical analysis 133
III. Results and discussion 134
1. Water binding capacity of soluble β-glucan 134
2. Fat binding capacity of soluble β-glucan 143
3. Bile acid binding capacity of soluble β-glucan 152
IV. Refernces 162
CHAPTER IV. Physicochemical characteristics and emulsion stabilities of modified barley β-glucan by n-octenyl succinic anhydride. 166
Abstract 167
I. Introduction 169
II. Materials and methods 172
1. Materials 172
2. Pre-treatment methods 172
3. Extraction and purification of barley β-glucan 174
4. Preparation of OSA-modified β-glucan 176
5. Physicochemical properties of OSA-modified β-glucan 178
6. Preparation and stability of OSA-modified β-glucan emulsions 180
7. Statistical analysis 182
III. Results and discussion 183
1. Degree of substitution and reaction efficiency of OSA-modified β-glucans 183
2. Molecular weight and viscosity of OSA-modified β-glucans 185
3. Droplet size and creaming stability of OSA-modified β-glucan emulsions 188
IV. References 203
CHAPTER V. Optimization of extraction conditions of soluble barley β-glucan by oxidative and heat treatmnet. 208
Abstract 209
I. Introduction 211
II. Materials and methods 214
1. Materials 214
2. Measurement of chemical compositions of the whole grain barleys 214
3. Determination of catalysts and inhibitors for Fenton reaction 216
4. Experimental design 217
5. Oxidative and heat treatmnet 218
6. Extraction of total and soluble β-glucan from barley solutions 218
7. Measurement of β-glucan purity 219
8. Statistical analysis 220
III. Results and discussion 222
1. Chemical compositions of whole grain barleys 222
2. Potential catalysts and inhibitors for oxidative degradation of β-glucans 224
3. Optimization of extraction conditions for soluble β-glucan 227
3.1. Experimental values 227
3.2. Model fitting 230
3.3. Interpretation of response surface models 235
3.4. Verification of predictive models 242
IV. References 245
CHAPTER VI. Conclusions 256
SUMMARY IN KOREAN 260