목차

Literature review 1
Ⅰ. Obesity 1
Ⅱ. Adipocyte and Adipose Tissue 3
Ⅲ. Type of Anti-obesity Drugs 15
Ⅳ. Turmeric (Curcuma longa L.) 17
Chapter Ⅰ. Curcuma longa L. Inhibited Adipogenesis in 3T3-L1 Cells
Abstract 18
1. Introduction 19
2. Materials 20
2.1. Plant Material 20
2.2. Cell Line 20
2.3. Chemical Reagents 20
3. Methods 21
3.1. Extraction of Curcuma longa L. 21
3.2. Determination of In vitro Anti-aipogenesis Activity Assay 21
3.2.1. Cell Culture and Adipocyte Differentiation 21
3.2.2. Cytotoxicity 22
3.2.3. Measurement of Lipid Accumulation Level 22
3.2.4. Measurement of Intracellular Triglyceride (TG) Level 22
3.2.5. Western Blotting Analysis 25
3.3. Statistical Analysis 26
4. Results and Discussion 26
4.1. In vitro Anti-aipogenesis Effects of CLM 26
4.1.1. Cytotoxicity 26
4.1.2. Lipid Accumulation Level of CLM 26
4.1.3. Intracellular TG Level of CLM 29
4.1.4. Expression of Transcription Factor 29
5. Conclusion 32
Chapter Ⅱ. Curcuma longa L. Enhanced Lipolysis in 3T3-L1 Cells and Reduced High-fat Diet-induced Animal Model
Abstract 33
1. Introduction 34
2. Materials 36
2.1. Plant Material 36
2.2. Cell Line 36
2.3. Animals and Diets 36
2.4. Chemical Reagents 37
3. Methods 39
3.1. Extraction of Curcuma longa L. 39
3.2. Determination of In vitro Lipolysis Activity Assay 39
3.2.1. Cell Culture and Adipocyte Differentiation 39
3.2.2. Cytotoxicity 41
3.2.3. Measurement of Lipid Accumulation Level 41
3.2.4. Measurement of Intracellular TG Level 41
3.2.5. Measurement of Free Glycerol Content 42
3.2.6. Quantitative Real-time Polymerase Chain Reaction 42
3.3. Determination of In vivo Toxicity 43
3.3.1. Experimental Groups 43
3.4. Determination of In vivo Anti-obese Activity 43
3.4.1. Experimental Groups 43
3.4.2. Assay for Serum Lipid Profiles 46
3.4.3. Quantitative Real-time Polymerase Chain Reaction 46
3.5. Statistical Analysis 46
4. Results and Discussion 46
4.1. In vitro Lipolysis Effects of CLM 46
4.1.1. Lipid Accumulation Level 46
4.1.2. Intracellular TG Level 46
4.1.3. Free Glycerol Content 47
4.1.4. Changes in mRNA Expression Level 51
4.2. In vivo Toxicity 53
4.2.1. Body Weight and Survial Rate 53
4.2.2. Organ Weights 53
4.3. In vivo Anti-obese Effect of High Fat-induced Mice 55
4.3.1. Body Weight and Food Intake 55
4.3.2. Organ Weights 55
4.3.3. Adipose Tissue Weights 56
4.3.4. Serum Lipid Profiles Levels 63
4.3.5. Changes in mRNA Expression Level 63
5. Conclusion 69
Chapter Ⅲ. Antioxidant Activity and Anti-obesity Effects from Curcuma longa L.
Abstract 70
1. Introduction 71
2. Materials 73
2.1. Plant Material 73
2.2. Cell Line 73
2.3. Chemical Reagents 73
3. Methods 74
3.1. Extraction of Curcuma longa L. 74
3.2. Analyses of Major and Minor Components 74
3.3. Measurement of Phenolic Compounds and Flavonoids Level 75
3.3.1. Phenolic Compounds Level 75
3.3.2. Flavonoids Level 75
3.4. Determination of Radical Scavenging Activity Assay 77
3.4.1. DPPH Radical Scavenging Activity Assay 77
3.4.2. ABTs Cation Radical Scavenging Activity Assay 77
3.5. Determination of In vitro Anti-obesity Activity Assay 77
3.5.1. Cell Culture and Adipocyte Differentiation 77
3.5.2. Cytotoxicity 78
3.5.3. Measurement of Lipid Accumulation Level 78
3.6. Statistical Analysis 79
4. Results and Discussion 79
4.1. Major and Minor Components of Curcuma longa L. 79
4.2. Yield of Extracts 79
4.3. Phenolic Compounds and Flavonoids Levels of Extracts 82
4.4. Radical Scavenging Activity of Extracts 82
4.4.1. DPPH Radical Scavenging Activity 82
4.4.2. ABTs Cation Radical Scavenging Activity 83
4.5. In vitro Anti-obesity Activity of Extracts 87
4.5.1. Cytotoxicity in 3T3-L1 Cells 87
4.5.2. Lipid Accumulation Levels of Extracts in 3T3-L1 Cells 87
5. Conclusion 90
References 91
Abstract in Korean 101
Acknowledgement 104