The nutritional and anti-microbial characteristics of bovine liver treated with supercritical carbon dioxide (SC-CO2) under different conditions were investigated and the data were compared to the treatment of organic solvents. The bovine liver was treated with SC-CO2 with 200~450 bar at 45 and 60℃ for 3 h or ether, mixture solvent of chloroform and methanol (2:1, v/v). Most of the mineral contents treated by SC-CO2 were higher than those of non-treated samples (control). High performance liquid chromatography (HPLC) analysis showed that vitamin A and B3 in the bovine liver were not dramatically affected by SC-CO2. The contaminated bovine liver was treated with SC-CO2 under various conditions such as treatment time (0.5~3 h) and pressure (50~400 bar), the colony forming units (CFU) of aerobic and coliform bacteria were decreased to 2.33 and 4.49 log CFU/g. In particular, coliform bacteria was completely inactivated by two SC-CO2 conditions, 400 bar, 2 h and 60℃ and 100 bar, 3 h and 60℃. SC-CO2 treatment was efficient in inactivating microorganism in the food containing a low moisture content, although aerobic bacteria did not be completely inactivated by SC-CO2 treatment . The yields of lipid extracted from the bovine liver by SC-CO2 were higher than those of organic solvents treatment. The rancidity using acid value (AV) and peroxide value (POV) was measured to compare the quality of extracted fat. As a result, AV and POV of fat extracted by SC-CO2 were higher than those of fat extracted by organic solvents. To confirm protein denaturation of the bovine liver by treatment of SC-CO2 or organic solvents, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on the liver samples was conducted. No denaturation of proteins was observed in the bovine liver treated by SC-CO2, while organic solvents occured denaturation of proteins. The contents of amino acids were higher in the order of 300, 200 and 450 bar at 60℃ and 450, 300 and 200 bar at 45℃. In particular, all samples contained high amounts of essential amino acids rich in animal proteins. Consequently, these results demonstrated that changes of nutritional composition, protein and fat in the bovine liver were decreased by SC-CO2. It was suggested that SC-CO2 treatment is very useful to produce high nutritional value-products without microbial contamination.