지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 소인섭
- 발행연도
- 2013
- 저작권
- 제주대학교 논문은 저작권에 의해 보호받습니다.
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초록· 키워드
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The aim of this study was to establish plant regeneration from leaf explants of globally rare and endangered species Sedum tosaense Makino.
The leaf explants of S. tosaense were cultured on the MS medium supplemented with different concentration of BA and NAA for plant regeneration. Callus induction and weight obtained the highest (100%) on MS medium containing 2.0 mg?L-1 BA and 1.0 mg?L-1 NAA. The highest number of shoots, weight of shoots, compactness of shoots were regeneration when callus were cultured on MS medium containing 2.0 mg?L-1 BA and 1.0 mg?L-1 NAA. The axillary bud were cultured on the MS media supplemented with combination of BA and NAA for in vitro mass propagation of S. tosaense . MS medium supplemented with 2.0 mg?L-1 BA and 1.0 mg?L-1 NAA was number of shoots 7.9, weight of shoots 66.9 mg by effective the best for mass propagation. For rooting, MS medium supplemented with and without 2.0 g?L-1 activated charcoal was tested. The optimal results were observed using MS medium supplemented with 2.0 g?L-1 activated charcoal, on which 85.7 (No. of root), length of root 4.6 cm. 1200 ppm CO2 and 350 ppm CO2 were supplide for make certain the effects of CO2 on pre-acclimatization by photoautotrophic culture. Number of leaf, leaf width, leaf thickness, length of shoot, length of root, total weight and chlorophyll contents of 1200 ppm CO2 treatment was estuimented higher than 350 ppm CO2 treatment. The survival rate was 100% in soil acclimatization of regenerated plantlets were mixed culture soil with peat moss and perlite that was the highest culture condition and the greated growth.
The leaf explants of S. tosaense were cultured on the MS medium supplemented with different concentration of BA and NAA for plant regeneration. Callus induction and weight obtained the highest (100%) on MS medium containing 2.0 mg?L-1 BA and 1.0 mg?L-1 NAA. The highest number of shoots, weight of shoots, compactness of shoots were regeneration when callus were cultured on MS medium containing 2.0 mg?L-1 BA and 1.0 mg?L-1 NAA. The axillary bud were cultured on the MS media supplemented with combination of BA and NAA for in vitro mass propagation of S. tosaense . MS medium supplemented with 2.0 mg?L-1 BA and 1.0 mg?L-1 NAA was number of shoots 7.9, weight of shoots 66.9 mg by effective the best for mass propagation. For rooting, MS medium supplemented with and without 2.0 g?L-1 activated charcoal was tested. The optimal results were observed using MS medium supplemented with 2.0 g?L-1 activated charcoal, on which 85.7 (No. of root), length of root 4.6 cm. 1200 ppm CO2 and 350 ppm CO2 were supplide for make certain the effects of CO2 on pre-acclimatization by photoautotrophic culture. Number of leaf, leaf width, leaf thickness, length of shoot, length of root, total weight and chlorophyll contents of 1200 ppm CO2 treatment was estuimented higher than 350 ppm CO2 treatment. The survival rate was 100% in soil acclimatization of regenerated plantlets were mixed culture soil with peat moss and perlite that was the highest culture condition and the greated growth.
목차
Ⅰ. 서 언 1Ⅱ. 재료 및 방법 41. 식물재료 42. 잎절편으로부터의 식물체 재분화 43. 대량증식 64. 기내발근유도 65. 광독립배양에 의한 순화전처리 86. 무균배양묘의 토양순화 8Ⅲ. 결과 및 고찰 91. 잎절편으로부터의 식물체 재분화 92. 대량증식 173. 기내발근유도 204. 광독립배양에 의한 순화전처리 235. 무균배양묘의 토양순화 27Ⅳ. 적 요 30Ⅴ. 참 고 문 헌 31Ⅵ. 감 사 의 글 38
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