지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 79 - 87 (9page)
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The aim of this study was to establish plant regeneration from leaf explants of Sedum tosaense Makino, which is globally rare and endangered species. The leaf explants of S. tosaense were cultured on the MS medium supplemented with different concentration of BA and NAA for callus induction. Callus induction was showed the highest (100%) on MS medium containing 2.0 mg․L-1 BA and 1.0 m g․L-1 NAA. The highest number of shoots were regenerated when callus were cultured on MS medium containing 2.0 m g․L-1 BA and 1.0 mg․L-1 NAA for 5 weeks. The axillary bud were cultured on the MS media supplemented with combination of BA and NAA for in vitro propagation. The highest number of adventitious shoot (7.9 per explants) formed at 1.0 mg․L-1 NAA and 2.0 mg․L-1 BA. For rooting, MS medium supplemented with or without 2.0 g․L-1 activated charcoal was tested. The optimal results were observed using MS medium supplemented with 2.0 g․L-1 activated charcoal, on which 85.7 (No. of root), 4.6 cm (length of root). 1,200 ppm CO2 and 350 ppm CO2 were supplied for make certain the effects of CO2 on pre-acclimatization by photoautotrophic culture. 1,200 ppm CO2 treatment was established higher than 350 ppm CO2 treatment. Soil acclimatization of in vitro plantlets was the best in mixture soil consisted of peat moss and perlite with 100% survival rate and they showed the maximum growth.
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참고문헌 (28)
참고문헌 신청
Anonymus, B / 2000 / Threatened wildlife of Japan -Red data book. Japan Wildlife research center / environment agency of Japan
Choi HK / 2002 / Mass production of paclitaxel by plant cell culture / Kor J Plant Biotech 29 : 59 ~ 62
Dumas E / 1995 / In vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants influence of activated charcoal. Plant Cell / Tissue and Org Cult
Eymar E / 2000 / Effect of activated charcoal and 6-benzyladenine on in vitro nitrogen uptake by Lagerstroemia indica. Plant Cell / Tissue and Organ Culture 63 : 57 ~ 65
He AM / 1998 / Hepatoprotective triterpenes from Sedum sarmentosum / Phytochemistry 49 : 2607 ~ 2610
Choi HK / 2002 / Mass production of paclitaxel by plant cell culture / Kor J Plant Biotech 29 : 59 ~ 62
Dumas E / 1995 / In vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants influence of activated charcoal. Plant Cell / Tissue and Org Cult
Eymar E / 2000 / Effect of activated charcoal and 6-benzyladenine on in vitro nitrogen uptake by Lagerstroemia indica. Plant Cell / Tissue and Organ Culture 63 : 57 ~ 65
He AM / 1998 / Hepatoprotective triterpenes from Sedum sarmentosum / Phytochemistry 49 : 2607 ~ 2610
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