지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 우홍정
- 발행연도
- 2013
- 저작권
- 경희대학교 논문은 저작권에 의해 보호받습니다.
이용수2
이 논문의 연구 히스토리 (2)
- 이 논문의 후속연구가 궁금하신가요?
- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
- 이 논문의 연구 히스토리 확인하기
초록· 키워드
오류제보하기
Objectives : This study was performed to elucidate the pro-apoptotic effect of Artemisiae capillaris herba extracted with ethanol on biliary tract cancer cells.
Materials and Methods : A biliary tract cancer cell line, SNU-1196 was used in this study. Cells were treated with different concentrations of Artemisiae capillaris herba for 24, 48 and 72 hours. After the treatment, cell viability, apoptosis, caspase activities and the mRNA expressions of the Bcl-2, Bax, P53, and P21 were measured by using MTT assay, cell cycle analysis, apoptosis assay, and RT-PCR. The cell cycle analysis was done by flow cytometry and apoptosis assay by cell death detection ELISA kit.
Results : Artemisiae capillaris herba inhibited proliferation of SNU-1196 in long-time culture group with dose-dependent manner. All cells treated with Artemisiae capillaris herba showed increased apoptosis with dose- and time-dependent manner. Exposure of SNU-1196 to Artemisiae capillaris herba induced caspase-3 activation. However, apeoptosis was blocked when SNU-1196 was treated together with the pancaspase inhibitor Z-VAD-FMK and the caspase-3 inhibitor Z-DEVE-FMK. After the treatment of Artemisiae capillaris herba, the mRNA expressions of caspase -3, -8, -9, p53, and p21 was increased in all cells. Artemisiae capillaris herba resulted in a significant decrease in Bcl-2 and an increase in Bax mRNA levels.
Conclusion : These results suggest that Artemisiae capillaris herba would be beneficial in the treatment of biliary tract cancer.
Materials and Methods : A biliary tract cancer cell line, SNU-1196 was used in this study. Cells were treated with different concentrations of Artemisiae capillaris herba for 24, 48 and 72 hours. After the treatment, cell viability, apoptosis, caspase activities and the mRNA expressions of the Bcl-2, Bax, P53, and P21 were measured by using MTT assay, cell cycle analysis, apoptosis assay, and RT-PCR. The cell cycle analysis was done by flow cytometry and apoptosis assay by cell death detection ELISA kit.
Results : Artemisiae capillaris herba inhibited proliferation of SNU-1196 in long-time culture group with dose-dependent manner. All cells treated with Artemisiae capillaris herba showed increased apoptosis with dose- and time-dependent manner. Exposure of SNU-1196 to Artemisiae capillaris herba induced caspase-3 activation. However, apeoptosis was blocked when SNU-1196 was treated together with the pancaspase inhibitor Z-VAD-FMK and the caspase-3 inhibitor Z-DEVE-FMK. After the treatment of Artemisiae capillaris herba, the mRNA expressions of caspase -3, -8, -9, p53, and p21 was increased in all cells. Artemisiae capillaris herba resulted in a significant decrease in Bcl-2 and an increase in Bax mRNA levels.
Conclusion : These results suggest that Artemisiae capillaris herba would be beneficial in the treatment of biliary tract cancer.
목차
Ⅰ. 緖 論 1Ⅱ. 方 法 21. 재료 21) 약재 22) 검액의 조제 23) 세포배양 및 시약 22. 방법 21) 세포 증식 분석 22) 세포 주기 분석 33) 세포자멸 분석 34) Caspase 활성도 분석 35) Caspase inhibitor를 투여한 후 세포 증식율 측정 46) 역전사 중합효소연쇄반응 47) 통계분석 6Ⅲ. 結 果 71. 茵蔯 추출물이 담도암세포주 세포증식에 미치는 영향 72. 茵蔯 추출물이 담도암세포주 세포주기에 미치는 영향 93. 茵蔯 추출물이 담도암세포주 세포자멸에 미치는 영향 104. 茵蔯 추출물에 의한 Caspase mRNA 발현의 변화 125. Caspase inhibitor가 茵蔯 추출물 처리 후의 세포활성에 미치는 영향 146. 茵蔯 추출물에 의한 Bcl-2, Bax mRNA 발현의 변화 167. 茵蔯 추출물에 의한 p53, p21 mRNA 발현의 변화 18Ⅳ. 考 察 20Ⅴ. 結 論 23Ⅵ. 參 考 文 獻 24ABSTRACT 27
최근 본 자료
댓글(0)
0