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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 587 - 598 (12page)
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Objectives : This study was performed to elucidate the pro-apoptotic effect of Artemisiae capillaris herba extracted with ethanol on biliary tract cancer cells.
Materials and Methods : A biliary tract cancer cell line, SNU-1196 was used in this study. Cells were treated with different concentrations of Artemisiae capillaris herba for 24, 48 and 72 hours. After the treatment, cell viability, apoptosis, caspase activities and the mRNA expressions of the Bcl-2, Bax, P53, and P21 were measured by using MTT assay, cell cycle analysis, apoptosis assay, and RT-PCR. The cell cycle analysis was done by flow cytometry and apoptosis assay by cell death detection ELISA kit. Results : Artemisiae capillaris herba inhibited proliferation of SNU-1196 in long-time culture group with dose-dependent manner. All cells treated with Artemisiae capillaris herba showed increased apoptosis with dose- and time-dependent manner. Exposure of SNU-1196 to Artemisiae capillaris herba induced caspase-3 activation. However, apeoptosis was blocked when SNU-1196 was treated together with the pancaspase inhibitor Z-VAD-FMK and the caspase-3 inhibitor Z-DEVE-FMK. After the treatment of Artemisiae capillaris herba, the mRNA expressions of caspase -3, -8, -9, p53, and p21 was increased in all cells. Artemisiae capillaris herba resulted in a significant decrease in Bcl-2 and an increase in Bax mRNA levels. Conclusions : These results suggest that Artemisiae capillaris herba would be beneficial in the treatment of biliary tract cancer.
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참고문헌 (30)
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/ / 중앙행정기관 - 보건복지부 - 암등록통계 - 61개 암종/성/연령(5세)별 암발생자수
Alijiffry M / 2009 / Advances in diagnosis, treatment and palliation of cholangiocarcinoma / World J Gastroenterol 15 : 4240 ~ 4262
Amaral JD / 2010 / The role of p53 in apoptosis / Discov Med 9 : 145 ~ 152
Hu YQ / 2000 / Apoptosis in Human Hepatoma Cell Line SMMC-7721 Induced by Water-soluble Macromolecular Components of Artemisia capillaris Thunberg / Jpn J Cancer Res 91 : 11
Itoh H / 2005 / Magnitude of combination therapy of radical resection and external beam radiotherapy for patients with carcinomas of the extrahepatic bile duct and gallbladde
Alijiffry M / 2009 / Advances in diagnosis, treatment and palliation of cholangiocarcinoma / World J Gastroenterol 15 : 4240 ~ 4262
Amaral JD / 2010 / The role of p53 in apoptosis / Discov Med 9 : 145 ~ 152
Hu YQ / 2000 / Apoptosis in Human Hepatoma Cell Line SMMC-7721 Induced by Water-soluble Macromolecular Components of Artemisia capillaris Thunberg / Jpn J Cancer Res 91 : 11
Itoh H / 2005 / Magnitude of combination therapy of radical resection and external beam radiotherapy for patients with carcinomas of the extrahepatic bile duct and gallbladde
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