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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology and Biomaterials 제48권 제3호
- 발행연도
- 2024.9
- 수록면
- 107 - 113 (7page)
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초록· 키워드
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The efficiency of protein extraction from Hongjam, a steamed mature silkworm, was quantitatively evaluated using various chemical buffers and physical methods. This study considers the difficulty of protein extraction yield due to the high content of hydrophobic amino acids in Hongjam compared to 5th instar-3rd day silkworm larvae. Results indicated that urea buffer enhanced protein yield more effectively than RIPA buffer. Additionally, the application of physical methods such as microwave treatment to samples treated with RIPA buffer increased yields by up to 22%, achieving concentrations as high as 3.9 mg/mL. Circular dichroism (CD) analysis showed that proteins extracted with urea buffer retained their structural integrity, exhibiting deeper and more prominent peaks associated with random coil structure. In addition, physical methods such as vortexing, sonication, microwave and homogenization increased the extraction yield of larger molecules without altering protein structures, suggesting their potential scalability for industrial applications. These results demonstrate the critical role of selecting appropriate extraction methods to optimize the yield and functionality of proteins from Hongjam, with implications for its use in biotechnological applications and nutraceuticals.
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