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논문 기본 정보

자료유형
학술저널
저자정보
Kim Dockyu (Division of Life Sciences, Korea Polar Research Institute, Incheon 21990, Republic of KoreaDepartment of Polar Sciences, University of Science and Technology, Incheon 21990, Republic of Kore) Kim Han-Woo (Division of Life Sciences, Korea Polar Research Institute, Incheon 21990, Republic of KoreaDepartment of Polar Sciences, University of Science and Technology, Incheon 21990, Republic of Kore) Lee Hyoungseok (Division of Life Sciences, Korea Polar Research Institute, Incheon 21990, Republic of KoreaDepartment of Polar Sciences, University of Science and Technology, Incheon 21990, Republic of Kore)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology Vol.34 No.9
발행연도
2024.9
수록면
1,867 - 1,875 (9page)
DOI
10.4014/jmb.2406.06021

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초록· 키워드

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Identification of the biochemical metabolic pathway for lignin decomposition and the responsible degradative enzymes is needed for the effective biotechnological valorization of lignin to renewable chemical products. In this study, we investigated the decomposition of kraft lignin by the soil bacterium Pseudomonas kribbensis CHA-19, a strain that can utilize kraft lignin and its main degradation metabolite, vanillic acid, as growth substrates. Gel permeation chromatography revealed that CHA-19 decomposed polymeric lignin and degraded dehydrodivanillin (a representative lignin model compound); however, the degradative enzyme(s) and mechanism were not identified. Quantitative polymerase chain reaction with mRNAs from CHA-19 cells induced in the presence of lignin showed that the putative genes coding for two laccase-like multicopper oxidases (LMCOs) and three dye-decolorizing peroxidases (DyPs) were upregulated by 2.0- to 7.9-fold compared with glucose-induced cells, which indicates possible cooperation with multiple enzymes for lignin decomposition. Computational homology analysis of the protein sequences of LMCOs and DyPs also predicted their roles in lignin decomposition. Based on the above data, CHA-19 appears to initiate oxidative lignin decomposition using multifunctional LMCOs and DyPs, producing smaller metabolites such as vanillic acid, which is further degraded via ortho- and meta-ring cleavage pathways. This study not only helps to better understand the role of bacteria in lignin decomposition and thus in terrestrial ecosystems, but also expands the biocatalytic toolbox with new bacterial cells and their degradative enzymes for lignin valorization.

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