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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.12
- 수록면
- 393 - 401 (9page)
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초록· 키워드
오류제보하기
Fire blight and black shoot blight are two major bacterial diseases that affect apple and pear production in Korea and are caused by Erwinia amylovora and E. pyrifoliae, respectively. These diseases have recently reached epidemic levels, heightening the risk of coinfection within orchards and even individual trees. Traditional detection methods often fail to distinguish between these pathogens due to their similar characteristics and disease symptoms creating an urgent need for improved detection tools. In this study, we developed a novel droplet digital polymerase chain reaction (ddPCR) technique that specifically quantifies E. amylovora, with a detection range of 10³ to 10<sup>7</sup> cfu/ml for cell culture templates and copies/ml for genomic DNA templates. The ddPCR platform is equipped with two fluorescence channels (FAM and HEX/VIC) and was further applied to develop a duplex ddPCR method for the simultaneous detection and absolute quantification of both pathogens. The method was tested on mixed DNA and cell cultures of E. amylovora and E. pyrifoliae and successfully quantified both pathogens within a single reaction. Moreover, duplex ddPCR effectively identified both pathogens in fruits artificially inoculated with various bacterial mixtures. This study provides valuable insights for addressing the cooccurrence of Erwinia diseases in orchards and offers a promising approach for precise diagnosis in plant disease management.
목차
Introduction
Materials and Methods
Results
Discussion
References
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