지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2023.2
- 수록면
- 141 - 148 (8page)
이용수
초록· 키워드
오류제보하기
Black shoot blight disease caused by Erwinia pyrifoliae has serious impacts on quality and yield in pear production in Korea; therefore, rapid and accurate methods for its detection are needed. However, traditional detection methods require a great deal of time and fail to achieve absolute quantification. In the present study, we developed a droplet digital polymerase chain reaction (ddPCR) method for the detection and absolute quantification of E. pyrifoliae using a pair of species-specific primers. The detection range was 103-107 copies/ml (DNA templates) and cfu/ml (cell culture templates). This new method exhibited good linearity and repeatability and was validated by absolute quantification of E. pyrifoliae DNA copies from samples of artificially inoculated immature pear fruits. Here, we present the first study of ddPCR assay for the detection and quantification of E. pyrifoliae. This method has potential applications in epidemiology and for the early prediction of black shoot blight outbreaks.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Discrimination and Detection of Erwinia amylovora and Erwinia pyrifoliae with a Single Primer Set
The Plant Pathology Journal
2022 .06
-
Development of a Duplex Droplet Digital Polymerase Chain Reaction Assay for Simultaneous Quantitative Detection of Two Erwinia Species
Research in Plant Disease(식물병연구)
2024 .12
-
Comparative Genome Analysis Reveals Natural Variations in the Genomes of Erwinia pyrifoliae, a Black Shoot Blight Pathogen in Apple and Pear
The Plant Pathology Journal
2020 .10
-
Evidence of Greater Competitive Fitness of Erwinia amylovora over E. pyrifoliae in Korean Isolates
The Plant Pathology Journal
2022 .08
-
Draft genome sequence of a bacterial plant pathogen Erwinia pyrifoliae strain EpK1/15 isolated from an apple twig showing black shoot blight
미생물학회지
2018 .03
-
Five Bacteriophages Shown Effective against Erwinia amylovora and E. pyrifoliae in South Korea
한국미생물학회 학술대회논문집
2022 .10
-
Characterization of the Lytic Bacteriophage phiEaP-8 Effective against Both Erwinia amylovora and Erwinia pyrifoliae Causing Severe Diseases in Apple and Pear
The Plant Pathology Journal
2018 .10
-
Uncovering Candidate Pathogenicity Genes in Erwinia pyrifoliae YKB12327 via Tn5-insertion Mutagenesis
Research in Plant Disease(식물병연구)
2024 .09
-
과수가지검은마름병 발생지 주변 장미과 식물의 분포 및 과수가지검은마름병 감수성 검정
Research in Plant Disease(식물병연구)
2024 .12
-
Effect of methemoglobin on carboxyhemoglobin (CO-Hb, %) quantification
한국분석과학회 학술대회
2023 .11
-
사과나무에서 가지검은마름병 억제를 위한 효율적 가지치기
Research in Plant Disease(식물병연구)
2016 .12
-
Development of Real-time Polymerase Chain Reaction Method for Rapid Detection and Quantification of Probiotics Based on Pan-genome Analysis
한국미생물학회 학술대회논문집
2022 .10
-
Whole-genome Comparison of Erwinia amylovora Strains that Caused a Fire Blight Outbreak in Korea
한국미생물학회 학술대회논문집
2017 .04
-
Detection of Erwinia amylovora and Analysis of Bacterial Diversity in Buried Fire Blight-diseased Plant and Soil
한국미생물학회 학술대회논문집
2021 .08
-
항혈청 기반 진단 스트립을 이용한 과수 화상병 현장진단
Research in Plant Disease(식물병연구)
2017 .12
-
Erwinia amylovora에 의한 팥배나무 화상병 발생 보고
Research in Plant Disease(식물병연구)
2023 .03
-
Comparison of Digital PCR and Quantitative PCR with Various SARS-CoV-2 Primer-Probe Sets
Journal of Microbiology and Biotechnology
2021 .01
-
Construction of Microbial Consortium FireFighter-C for Controlling Fire Blight Disease and Its Control Effect
Research in Plant Disease(식물병연구)
2024 .12
-
An advanced tool, droplet digital PCR (ddPCR), for absolute quantification of the red-tide dinoflagellate, Cochlodinium polykrikoides Margalef (Dinophyceae)
Algae
2017 .01
-
Survival of Erwinia amylovora on Surfaces of Materials Used in Orchards
Research in Plant Disease(식물병연구)
2019 .06
이 논문의 저자 정보
이 논문과 함께 이용한 논문
Characterization of the Lytic Bacteriophage phiEaP-8 Effective against Both Erwinia amylovora and Erwinia pyrifoliae Causing Severe Diseases in Apple and Pear
Jungkum Pa
,
k
,
Gyu Min Lee
외 4명
The Plant Pathology Journal
2018
.10
사과나무 잎과 줄기 내 가지마름병균(Erwinia pyrifoliae)의 분포양상
이영기
,
김경님
,
전금화
외 2명
한국농약과학회 학술발표대회 논문집
2020
.11
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2023-481-001553256