지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.5
- 수록면
- 314 - 328 (15page)
이용수
초록· 키워드
오류제보하기
The mechanism for the elimination of xenobiotics undergoes three different phases of reactions in organisms. Among these, glutathione S-transferases (GSTs) are classified as phase II detoxification enzymes, catalyzing the conjugation of electrophilic substrates to glutathione or reduced hydroperoxides. This study aimed to investigate the molecular characteristics, detoxification functions, and immune responses of GST omega (LhGSTO1) and kappa (LhGSTK1) in redlip mullet. The open reading frames of LhGSTO1 (720 bp) and LhGSTK1 (687 bp) encoded proteins of 239 and 228 amino acids, respectively. Sequence analysis revealed that LhGSTO1 and LhGSTK1 possessed GSH-binding sites in their N-terminal domains. Substrate-binding sites in the C-terminal domain were exclusively identified in LhGSTO1. In the tissue-specific transcription profile analysis, both LhGSTO1 and LhGSTK1 were ubiquitously expressed in all tissues of healthy mullets. Temporal expression analysis of LhGSTO1 and LhGSTK1 in the blood showed that their expression was significantly modulated by polyinosinic:polycytidylic (poly I:C), lipopolysaccharide (LPS), and Lactococcus garvieae. Different chemical and cellular assays were performed to assess the detoxification and cellular protective abilities of the two proteins. A substrate specificity test using the recombinant proteins revealed that both proteins possessed specific activity toward 1-chloro-2,4-dinitrobenzene (CDNB). In the disk diffusion assay, the smallest clearance zones were observed for LhGSTO1 and LGSTK1 against CdCl2. In the cell protection assay, both LhGSTO1 and LhGSTK1 showed significant Cd detoxification ability compared to the control. Collectively, these results demonstrate that GST omega and kappa are involved in host defense against immune stimulants and xenobiotics in redlip mullet.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Molecular characterization and isolation of a glutathione S-transferase kappa 1 from redlip mullet (Liza hematocheilus)
한국수산과학회 양식분과 학술대회
2022 .10
-
Molecular characterization, transcriptional analysis of interleukin 6 (IL-6) orthologue from Redlip mullet, Liza haematocheila
한국수산과학회 양식분과 학술대회
2019 .05
-
Galectin-1 from redlip mullet Liza haematocheilia: identification, immune responses, and functional characterization as pattern recognition receptors (PRRs) in host immune defense system
Fisheries and Aquatic Sciences
2022 .11
-
Molecular Insight and transcriptional analysis of the Transforming Growth Factorbeta 1 (TGF-β1) of Redlip Mullet Liza haematocheila
한국수산과학회 양식분과 학술대회
2019 .05
-
Identification of NOD-1 gene from redlip mullet (Liza haematocheilus): Molecular characterization and functional evaluation
한국수산과학회 양식분과 학술대회
2022 .10
-
Molecular and Transcriptional insight into glutathione S-transferase omega from Red Lip Mullet (Chelon haematocheilus)
한국수산과학회 양식분과 학술대회
2018 .05
-
Identification and molecular characterisation of WNT1 ligand in redlip mullet Planiliza haematocheila
한국수산과학회 양식분과 학술대회
2021 .04
-
Immune and xenobiotic responses of Glutathione S-Transferase theta (GST- Θ)disk abalone (Haliotis discus discus): with molecular characterization and functional analysis
한국수산과학회 양식분과 학술대회
2019 .05
-
Molecular characterization and expression analysis of interferon regulatory factor 2 in mullet, Liza haemotocheila
한국수산과학회 양식분과 학술대회
2019 .05
-
Comparative Genomics in Brassicaceae Family : Analysis of 434 Glutathione S-Transferases (GST) Genes and Their Evolutionary Analysis
한국원예학회 학술발표요지
2016 .05
-
Detoxification of Tebupirimfos in Soil of Korean Ginseng Field by Human Recombinant Glutathione Stransferase M2-2
한국식품영양과학회 학술대회발표집
2018 .10
-
MOLECULAR INSIGHTS OF INTERFERON-INDUCED GTP-BINDING PROTEIN, MX GENE IN MULLET Liza haemotocheilus
한국수산과학회 양식분과 학술대회
2018 .05
-
A TELEOST SPECIFIC GLUTATHIONE S-TRANSFERASE FAMILY PROTEIN FROM BLACK ROCKFISH : MOLECULAR AND FUNCTIONAL CHARACTERIZATION
한국수산과학회 양식분과 학술대회
2015 .05
-
Detox juice modulates detoxification enzymes and inflammatory cytokines under co-culture of multiple cells
한국식품영양과학회 학술대회발표집
2015 .08
-
Analysis of Transgenic Chinese Cabbage with Glutathione S- transferase (GST ) RNAi Vector to Induce a Large Quantity of PhenylethylIsothiocyanate
한국원예학회 학술발표요지
2015 .05
-
Bioaccumulation, alterations of metallothionein, and antioxidant enzymes in the mullet Mugil cephalus exposed to hexavalent chromium
Fisheries and Aquatic Sciences
2016 .01
-
Interferon regulatory factor 8 in mullet, Liza haemotocheila: Structural and functional evidence for its immunomodulatory function in teleost
한국수산과학회 양식분과 학술대회
2022 .10
-
Interferon regulatory factor 8 in mullet, Liza haemotocheila: Structural and functional evidence for its immunomodulatory function in teleost
한국수산과학회 양식분과 학술대회
2022 .10
-
Identification of Glutathione S-transferases kappa from big-belly seahorse (Hippocampus abdominalis): Investigation of its functional properties and post immune responses upon pathogenic stress
한국수산과학회 양식분과 학술대회
2017 .05
-
Gene Functional Studies Related to Heavy Metal Detoxification Using Transient Expression Assay of Plant Protoplasts
한국토양비료학회 학술발표회 초록집
2015 .12
최근 본 자료
댓글(0)
0