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논문 기본 정보

자료유형
학술저널
저자정보
Kim Yujin (Department of Dermatology, College of Medicine, Chung-Ang University, Seoul 06974, Republic of KoreaDepartment of Medicine, Graduate School, Chung-Ang University, Seoul 06973, Republic of Ko) Lee Jung Ok (Department of Dermatology, College of Medicine, Chung-Ang University, Seoul 06974, Republic of Korea) Lee Mun-Hoe (Health Food Research and Development, NEWTREE Co., Ltd., Seoul 05604, Republic of Korea) Kim Hyeong-Min (Health Food Research and Development, NEWTREE Co., Ltd., Seoul 05604, Republic of Korea) Chung Hee-Chul (Health Food Research and Development, NEWTREE Co., Ltd., Seoul 05604, Republic of Korea) Kim Do-Un (Health Food Research and Development, NEWTREE Co., Ltd., Seoul 05604, Republic of Korea) Lee Jin-Hee (Health Food Research and Development, NEWTREE Co., Ltd., Seoul 05604, Republic of Korea)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology Vol.34 No.1
발행연도
2024.1
수록면
17 - 28 (12page)
DOI
10.4014/jmb.2308.08013

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초록· 키워드

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Low molecular weight collagen peptide (LMWCP) is a collagen hydrolysate derived from fish. We investigated the effects of LMWCP on hair growth using human dermal papilla cells (hDPCs), human hair follicles (hHFs), patch assay, and telogenic C57BL/6 mice, while also examining the underlying mechanisms of its action. LMWCP promoted proliferation and mitochondrial potential, and the secretion of hair growth-related factors, such as EGF, HB-EGF, FGF-4, and FGF-6 in hDPCs. Patch assay showed that LMWCP increased the neogeneration of new HFs in a dose-dependent manner. This result correlated with an increase in the expression of dermal papilla (DP) signature genes such as, ALPL, SHH, FGF7, and BMP-2. LMWCP upregulated phosphorylation of glycogen synthase kinase-3β (GSK-3β) and β-catenin, and nuclear translocation of β-catenin, and it increased the expression of Wnt3a, LEF1, VEGF, ALP, and β-catenin. LMWCP promoted the growth of hHFs and increased the expression of β-catenin and VEGF. Oral administration of LMWCP to mice significantly stimulated hair growth. The expression of Wnt3a, β-catenin, PCNA, Cyclin D1, and VEGF was also elevated in the back skin of the mice. Furthermore, LMWCP increased the expression of cytokeratin and Keratin Type I and II. Collectively, these findings demonstrate that LMWCP has the potential to increase hair growth via activating the Wnt/β-catenin signaling pathway.

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