지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
A lateral flow immunoassay kit based on antigen-antibody interactions was developed to detect residues of beta-lactams, quinolones, tetracyclines, and sulfonamides in farmed fish. Group-specific antibodies showing cross-reactivity with other antibiotics in the same group were produced in rabbits. The rabbits were immunized eight times to obtain the maximum titers. Antibodies were extracted from the antisera collected from the immunized rabbits and produced group-specific reactions with antibiotics from the four groups. A kit was prepared that optimize conditions for the antigen-antibody reaction, using colloidal gold conjugated antibodies, and was designed to detect the four groups of antibiotics simultaneously. The kit enabled the detection of antibiotics in the four groups at below maximum residue limits (MRLs), which were 200 μg/kg for tetracyclines, 100 μg/kg for sulfonamides, 50 μg/kg for beta-lactams, and 100 μg/kg for quinolones. The cross-reactivity of the antibodies ranged from 10-80% for the sulfonamides, 20- 100% for tetracyclines, 38-100% for quinolones, and 20-100% for the beta-lactams, confirming that the antibodies were group specific. The test kit was used 30 times to examine spiked antibiotics at the limits of detection (LODs) and all produced positive results, indicating high sensitivity. The LODs for the assay ranged from 4-20 ng/mL for beta-lactams, 25-50 ng/mL for sulfonamides, 20-100 ng/mL for tetracyclines, and 30-80 ng/mL for quinolones, and there were no false negative reactions at above these LODs. In addition, all of the LODs of the developed kit were correlated with high-performance liquid chromatography (HPLC) data. Our lateral flow immunoassay kit can simultaneously detect antibiotic residues from a large number of fish samples rapidly, strengthening the safety of domestic farmed and imported fish.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Development of cellulose nano beads based a rapid detection kit to detect staphylococcal enterotoxin B
Korean Journal of Agricultural Science
2019 .09
-
퇴비 내 잔류 항생제 농도의 시간적 변화 분석
한국토양비료학회 학술발표회 초록집
2021 .11
-
Tetracycline residues in retailed pork meat and liver in public markets of Davao city, Philippines
Food Science and Preservation
2024 .12
-
Prevalence of Antibiotic Residues and Antibiotic Resistance in Isolates of Chicken Meat in Korea
한국축산식품학회지
2018 .01
-
A Study on the Validation of the Matrix Effect Kit for Analysis of Pesticide Residue
한국식품영양과학회 학술대회발표집
2024 .10
-
토양 중 유기물 함량이 고추의 항생제 전이에 미치는 영향
한국토양비료학회 학술발표회 초록집
2017 .05
-
Remove of Three Pathogenic Bacteria in Cultured Fish and Tetracycline Antibiotics by Using Underwater Non-Thermal Dielectric Barrier Discharge Plasma
한국수산과학회 양식분과 학술대회
2022 .10
-
가축 항생제 처리 수준에 따른 사료작물의 생장 억제 및 항생제 축적 차이
한국토양비료학회 학술발표회 초록집
2022 .10
-
항생물질이 배추 및 상추의 발아율에 미치는 영향
한국토양비료학회 학술발표회 초록집
2020 .10
-
가축분 퇴비 시용 농경지 내 Sulfonamides 내성균 분포
한국토양비료학회 학술발표회 초록집
2015 .12
-
Monitoring of Veterinary Antibiotics in the Agro-Environment
한국토양비료학회지
2019 .11
-
Distribution of Tetracyclines Resistant Bacteria and Resistance Genes in the Soil Fertilized with Livestock Manure Compost
한국토양비료학회 학술발표회 초록집
2015 .05
-
Milk yield and kit development of four breeds of rabbit in Ibadan, Nigeria
한국축산학회지
2017 .01
-
Development of Citrus Genetic Hybrids Detection Kit
한국원예학회 학술발표요지
2015 .10
-
High frequency of colonization by extended-spectrum beta-lactamase-producing Gram-negative bacilli in hemodialysis patients and their household contacts: dissemination between community and hospital
Epidemiology and Health
2022 .01
-
Residue Assessment of Veterinary Antibiotics in Manure-based Composts by Different Stages of Compost Production
한국농약과학회 학술발표대회 논문집
2021 .05
-
Monitoring of Seasonal Variation Characteristics for Veterinary Antibiotics in Agricultural Environment
한국토양비료학회지
2023 .02
-
Development of Rapidly Detection Kit Poisoning to Organophosphate Pesticide
한국농약과학회 학술발표대회 논문집
2017 .04
-
한우 인공수정에서 수정적기 진단키트 활용이 수태율에 미치는 영향
한국수정란이식학회지
2017 .01
-
가축분 퇴비에 존재하는 Tetracyclines의 저항성 미생물 우점종 분포 특성
한국토양비료학회 학술발표회 초록집
2019 .08
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-151-24-02-090183210