Effects of CTHRC1 on odontogenic differentiation and angiogenesis in human dental pulp stem cells

Kim Jong-soon; Lee Bin-Na; Chang Hoon-Sang; Hwang In-Nam; Oh Won-Mann; Hwang Yun-Chan

doi:10.5395/rde.2023.48.e18

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저자정보: Kim Jong-soon (Department of Conservative Dentistry School of Dentistry Chonnam National University Gwangju Korea.) Lee Bin-Na (Department of Conservative Dentistry School of Dentistry Chonnam National University Gwangju Korea.) Chang Hoon-Sang (Department of Conservative Dentistry School of Dentistry Chonnam National University Gwangju Korea.) Hwang In-Nam (Department of Conservative Dentistry School of Dentistry Chonnam National University Gwangju Korea.) Oh Won-Mann (Department of Conservative Dentistry School of Dentistry Chonnam National University Gwangju Korea.) Hwang Yun-Chan (Department of Conservative Dentistry School of Dentistry Chonnam National University Gwangju Korea.)

저널정보: 대한치과보존학회 Restorative Dentistry and Endodontics Restorative Dentistry and Endodontics 제48권 제2호

발행연도: 2023.5

수록면: 1 - 10 (10page)

DOI: 10.5395/rde.2023.48.e18

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Objectives This study aimed to determine whether collagen triple helix repeat containing-1 (CTHRC1), which is involved in vascular remodeling and bone formation, can stimulate odontogenic differentiation and angiogenesis when administered to human dental pulp stem cells (hDPSCs). Materials and Methods The viability of hDPSCs upon exposure to CTHRC1 was assessed with the WST-1 assay. CTHRC1 doses of 5, 10, and 20 µg/mL were administered to hDPSCs. Reverse-transcription polymerase reaction was used to detect dentin sialophosphoprotein, dentin matrix protein 1, vascular endothelial growth factor, and fibroblast growth factor 2. The formation of mineralization nodules was evaluated using Alizarin red. A scratch wound assay was conducted to evaluate the effect of CTHRC1 on cell migration. Data were analyzed using 1-way analysis of variance followed by the Tukey post hoc test. The threshold for statistical significance was set at p < 0.05. Results CTHRC1 doses of 5, 10, and 20 µg/mL had no significant effect on the viability of hDPSCs. Mineralized nodules were formed and odontogenic markers were upregulated, indicating that CTHRC1 promoted odontogenic differentiation. Scratch wound assays demonstrated that CTHRC1 significantly enhanced the migration of hDPSCs. Conclusions CTHRC1 promoted odontogenic differentiation and mineralization in hDPSCs.

#Collagen triple helix repeat containing-1 #Odontogenic #Mineralization #Dental pulp stem cell

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