Laser-Structured Si and PLGA Inhibit the Neuro2a Differentiation in Mono- and Co-Culture with Glia

Angelaki Despoina; Kavatzikidou Paraskevi; Fotakis Costas; Stratakis Emmanuel; Ranella Anthi

doi:10.1007/s13770-022-00497-7

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자료유형: 학술저널

저자정보: Angelaki Despoina (Institute of Electronic Structure and Laser Foundation for Research and Technology- Hellas (IESL- FORTH)) Kavatzikidou Paraskevi (Institute of Electronic Structure and Laser Foundation for Research and Technology- Hellas (IESL- FORTH)) Fotakis Costas (Institute of Electronic Structure and Laser Foundation for Research and Technology- Hellas (IESL- FORTH)) Stratakis Emmanuel (Institute of Electronic Structure and Laser Foundation for Research and Technology- Hellas (IESL- FORTH)) Ranella Anthi (Institute of Electronic Structure and Laser Foundation for Research and Technology- Hellas (IESL- FORTH))

저널정보: 한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제20권 제1호

발행연도: 2023.2

수록면: 111 - 125 (15page)

DOI: 10.1007/s13770-022-00497-7

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Background: The first step towards a successful neural tissue engineering therapy is the development of an appropriate scaffold and the in vitro study of the cellular response onto it. Methods: Here, we fabricated nano- and micro- patterned Si surfaces via direct ultrafast laser irradiation, as well as their replicas in the biodegradable poly(lactide-co-glycolide), in order to use them as culture substrates for neuronal cells. The differentiation of neuro2a cells on the Si platforms and their replicas was studied both in a mono-culture and in a co-culture with glial cells (Schwann—SW10). Results: It was found that the substrate’s roughness inhibits the differentiation of the neuronal cells even in the presence of the differentiation medium, and the higher the roughness is, the more the differentiation gets limited. Conclusion: Our results highlight the importance of the substrate’s topography for the controlled growth and differentiation of the neuronal cells and their further study via protein screening methods could shed light on the factors that lead to limited differentiation; thus, contributing to the long standing request for culture substrates that induce cells to differentiate.

#Neuronal differentiation Glia-neurons co-culture Nano/micro topography Laser structuring Neural tissue engineering

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