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논문 기본 정보

자료유형
학술저널
저자정보
Yunyun Wei (School of Radiology The Second Afliated Hospital of Shandong First Medical University) Jianfeng Qiu (School of Radiology Shandong First Medical niversity and Shandong Academy of Medical Sciences) Ziqiang Han (School of Laboratory Animal & Shandong Laboratory Animal Center Shandong First Medical University & Shandong Academy of Medical Sciences) Xuanyi Wang (Department of Clinical Medicine Shandong First Medical University and Shandong Academy of Medical Sciences) Hui Zhang (School of Laboratory Animal & Shandong Laboratory Animal Center Shandong First Medical University & Shandong Academy of Medical Sciences) Xinya Hou (Department of Clinical Medicine Shandong First Medical University and Shandong Academy of Medical Sciences) Xiangwei Lv (Department of Clinical Medicine Shandong First Medical University and Shandong Academy of Medical Sciences) Xiaolong Mao (School of Laboratory Animal & Shandong Laboratory Animal Center Shandong First Medical University & Shandong Academy of Medical Sciences)
저널정보
한국생체재료학회 생체재료학회지 생체재료학회지 제27권
발행연도
2023.3
수록면
2,281 - 2,296 (16page)
DOI
https://doi.org/10.1186/s40824-023-00443-1

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BackgroundFor some ICU patients, an artificial airway must be established with an endotracheal tube, but Candida albicans can easily adhere to the tube surface and form a biofilm, leading to potentially life threatening fungal infections. Therefore, it is urgent to prevent and reduce C. albicans infections introduced by the endotracheal tube. However, there are few antifungal drugs effective against C. albicans, and each of these drugs may have adverse effects on human cells. Saccharomyces boulardii is regarded as an alternative strategy to inhibit the adhesion of C. albicans, but it is affected by environmental stress. We hypothesized that it is feasible to strengthen the antagonistic ability of S. boulardii via encapsulating and genetically modification. MethodsIn this study, a bioactive material carrying the overexpressed MCP1 gene of Saccharomyces boulardii was constructed based on one-step photo-crosslinking. This material achieved spatial growth control of S. boulardii by encapsulating each S. boulardii cell within a hydrogel pore. The bioactive material was coated on an endotracheal tube and tested for its ability to inhibit the adhesion of C. albicans. Additionally, the material’s antagonistic activity towards C. albicans was evaluated by detecting intracellular Adenosine-triphosphate content, reactive oxygen species level and the activity of antioxidative enzymes. Tissue invasion experiment was executed to further evaluate the anti-adhesion ability of S. boulardii bio-coating. ResultsEncapsulating the overexpression of MCP1 by S. boulardii in hydrogel pores enhanced the viability of probiotics in the presence of high salt and oxidation stress. When used as the coating of an endotracheal tube, the S. boulardii bioactive material efficiently inhibited the adhesion of C. albicans by impairing the activities of superoxide dismutase and catalase and disturbing mitochondrial functions. In vivo, the S. boulardii bioactive material coating displayed good biocompatibility and reduced the host tissue invasion and virulence of C. albicans. ConclusionsThe integration of genetic modification and immobilization model breaks the bottleneck of previous application of microorganisms, and provides a new way to prevent fungal infections introduced by endotracheal tubes.
#Medical device coating #Fungal infection #Probiotics #Antagonism #Hydrogel

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