Intestinal segment and vitamin D3 concentration affect gene expression levels of calcium and phosphorus transporters in broiler chickens

Jincheng Han; Lihua Wu; Xianliang Lv; Mengyuan Liu; Yan Zhang; Lei He; Junfang Hao; Li Xi; Hongxia Qu; Chuanxin Shi; Zhiqiang Li; Zhixiang Wang; Fei Tang; Yingying Qiao

추천

검색

자료유형: 학술저널

저자정보: Jincheng Han (Shangqiu Normal University) Lihua Wu (Shangqiu Normal University) Xianliang Lv (Shangqiu Normal University) Mengyuan Liu (Shangqiu Normal University) Yan Zhang (Shangqiu Normal University) Lei He (Shangqiu Normal University) Junfang Hao (Shangqiu Normal University) Li Xi (Shangqiu Normal University) Hongxia Qu (Shangqiu Normal University) Chuanxin Shi (Shangqiu Normal University) Zhiqiang Li (Shangqiu Normal University) Zhixiang Wang (Henan Agricultural University) Fei Tang (Shandong Haineng Bioengineering Co. Ltd.) Yingying Qiao (Sumy National Agrarian University)

저널정보: 한국축산학회(구 한국동물자원과학회) 한국축산학회지 한국축산학회지 제65권 제2호

발행연도: 2023.3

수록면: 336 - 350 (15page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Two experiments were conducted in this research. Experiment 1 investigated the spatial expression characteristics of calcium (Ca) and phosphorus (P) transporters in the duodenum, jejunum, and ileum of 21-day-old broilers provided with adequate nutrient feed. Experiment 2 evaluated the effects of dietary vitamin D3 (VD3) concentration (0, 125, 250, 500, 1,000, and 2,000 IU/kg) on growth performance, bone development, and gene expression levels of intestinal Ca and P transporters in 1–21-day-old broilers provided with the negative control diet without supplemental VD3. Results in experiment 1 showed that the mRNA levels of calcium- binding protein 28-kDa (CaBP-D28k), sodium-calcium exchanger 1 (NCX1), plasma membrane calcium ATPase 1b (PMCA1b), and IIb sodium-phosphate cotransporter (NaPi-IIb) were the highest in the broiler duodenum. By contrast, the mRNA levels of inorganic phosphate transporter 1 (PiT-1) and 2 (PiT-2) were the highest in the ileum. Results in experiment 2 showed that adding 125 IU/kg VD3 increased body weight gain (BWG), feed intake (FI), bone weight, and percentage and weight of Ca and P in the tibia and femur of 1–21-day-old broilers compared with the negative control diet (p < 0.05). The rise in dietary VD3 levels from 125 to 1,000 IU/kg further increased the BWG, FI, and weights of the bone, ash, Ca, and P (p < 0.05). No difference in growth rate and leg bone quality was noted in the broilers provided with 1,000 and 2,000 IU/kg VD3 (p > 0.05). Supplementation with 125–2,000 IU/kg VD3 increased the mRNA abundances of intestinal Ca and P transporters to varying degrees. The mRNA level of CaBP-D28k increased by 536, 1,161, and 28 folds in the duodenum, jejunum, and ileum, respectively, after adding 1,000 IU/kg VD3. The mRNA levels of other Ca and P transporters (PMCA1b, NCX1, NaPi-IIb, PiT-1, and PiT-2) increased by 0.57–1.74 folds by adding 1,000–2,000 IU/kg VD3. These data suggest that intestinal Ca and P transporters are mainly expressed in the duodenum of broilers. Moreover, the addition of VD3 stimulates the two mineral transporter transcription in broiler intestines.

#Vitamin D3 #Broiler chicken #CaBP-D28k #PMCA1b #NaPi-IIb #PiT-1

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!