지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2023.6
- 수록면
- 1,218 - 1,231 (14page)
- DOI
- 10.1038/s12276-023-01009-w
이용수
초록· 키워드
오류제보하기
The signaling pathways governing acetaminophen (APAP)-induced liver injury have been extensively studied. However, little is known about the ubiquitin-modifying enzymes needed for the regulation of APAP-induced liver injury. Here, we examined whether the Pellino3 protein, which has E3 ligase activity, is needed for APAP-induced liver injury and subsequently explored its molecular mechanism. Whole-body Peli3−/− knockout (KO) and adenovirus-mediated Peli3 knockdown (KD) mice showed reduced levels of centrilobular cell death, infiltration of immune cells, and biomarkers of liver injury, such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST), upon APAP treatment compared to wild-type (WT) mice. Peli3 deficiency in primary hepatocytes decreased mitochondrial and lysosomal damage and reduced the mitochondrial reactive oxygen species (ROS) levels. In addition, the levels of phosphorylation at serine 9 in the cytoplasm and mitochondrial translocation of GSK3β were decreased in primary hepatocytes obtained from Peli3−/− KO mice, and these reductions were accompanied by decreases in JNK phosphorylation and mitochondrial translocation. Pellino3 bound more strongly to GSK3β compared with JNK1 and JNK2 and induced the lysine 63 (K63)-mediated polyubiquitination of GSK3β. In rescue experiments, the ectopic expression of wild-type Pellino3 in Peli3−/− KO hepatocytes restored the mitochondrial translocation of GSK3β, but this restoration was not obtained with expression of a catalytically inactive mutant of Pellino3. These findings are the first to suggest a mechanistic link between Pellino3 and APAP-induced liver injury through the modulation of GSK3β polyubiquitination.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Pellino1 promotes chronic inflammatory skin disease via keratinocyte hyperproliferation and induction of the T helper 17 response
Experimental and Molecular Medicine
2020 .09
-
Inactivation of Sirtuin2 protects mice from acetaminophen-induced liver injury: possible involvement of ER stress and S6K1 activation
BMB Reports
2019 .01
-
Lysophosphatidic acid protects against acetaminophen-induced acute liver injury
Experimental and Molecular Medicine
2017 .01
-
Enhancement of paclitaxel-induced breast cancer cell death via the glycogen synthase kinase-3
BMB Reports
2016 .01
-
Heterogeneity in Liver Histopathology Is Associated With GSK-3β Activity and Mitochondrial Dysfunction in End-Stage Diabetic Rats on Differential Diets
BMB Reports
2020 .01
-
Techniques for investigating mitochondrial gene expression
BMB Reports
2020 .01
-
The estrogen-related receptor γ modulator, GSK5182, inhibits osteoclast differentiation and accelerates osteoclast apoptosis
BMB Reports
2021 .01
-
Molecular cross talkamongthecomponentsofthe regulatory machinery of mitochondrial structure and quality control
Experimental and Molecular Medicine
2020 .01
-
The coordinated regulation of mitochondrial structure and function by Drp1 for mitochondrial quality surveillance
BMB Reports
2019 .01
-
Current Understanding of Stem Cell and Secretome Therapies in Liver Diseases
조직공학과 재생의학
2017 .01
-
Application of Mitochondrial Transplantation Method in Various Indications
한국생물공학회 학술대회
2024 .09
-
Mitochondrial dysfunction suppresses p53 expression via calcium-mediated nuclear factor-kB signaling in HCT116 human colorectal carcinoma cells
BMB Reports
2018 .01
-
Hepatocyte organoids and cell transplantation: What the future holds
Experimental and Molecular Medicine
2021 .10
-
Metformin ameliorates bile duct ligation-induced acute hepatic injury via regulation of ER stress
BMB Reports
2020 .01
-
Effects of microRNA-135a on the epithelial–mesenchymal transition, migration and invasion of bladder cancer cells by targeting GSK3β through the Wnt/β-catenin signaling pathway
Experimental and Molecular Medicine
2018 .01
-
Emerging perspectives on mitochondrial dysfunction and inflammation in Alzheimer’s disease
BMB Reports
2020 .01
-
microRNA-200a-3p enhances mitochondrial elongation by targeting mitochondrial fission factor
BMB Reports
2017 .01
-
AGS위암세포에서 AMPK/Akt/GSK-3β 신호경로를 통한 삼채추출물의 항암효과
KSBB Journal
2018 .12
-
Liver Organoids: Formation Strategies and Biomedical Applications
조직공학과 재생의학
2021 .01
-
Ceramide kinase-mediated C1P metabolism attenuates acute liver injury by inhibiting the interaction between KEAP1 and NRF2
Experimental and Molecular Medicine
2024 .04
이 논문의 저자 정보
최근 본 자료
댓글(0)
0