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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2023.6
- 수록면
- 100 - 111 (12page)
이용수
초록· 키워드
오류제보하기
Plasmid-based expression vectors, the most useful genetic tool in recombinant DNA technology, carry the foreign gene into the cell, and keep it stable, thereby enabling its expression within cells. These vectors typically have a replication origin, selection marker, and promoter as main genetic parts. Each of them enables chromosome-independent replication within cells, exerts pressure for the selection of recombinant cells, and controls the expression of carried foreign genes. Many studies have reported to design and improve vectors by using the targeted replacement and/or mutagenesis to the aforementioned major parts and spanning sequence. Despite the consistent reports on expression vectors with new functions, such approach has some constraints of using unstandardized genetic parts limited in functional sequence space. As an alternative, synthetic biology provides a foundation for tailor-made expression systems by rational design and modification via assembling various modular parts. Nevertheless, due to the limited number of modular parts and the lack of diversity of vector scaffold that offer the basis for assembly of these components, it falls short of constructing the expression systems feasible to graft into getting more useful expression system. This review will give a brief overview of the three major components and current status of vector design for expression of foreign genes, and then discuss the prospect of developing expression systems based on the systematic approaches, consequently expecting to understood the new platform for construction and improvement on target-oriented expression vectors via assembly of functional modules and scaffolds.
목차
Abstract
1. INTRODUCTION
2. 발현벡터 주요부품과 특성개량
3. 시스템/합성 생물학 기반의 발현벡터 개발전략
4. CONCLUSION
REFERENCES
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