지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.12
- 수록면
- 490 - 498 (9page)
이용수
초록· 키워드
오류제보하기
Panicle blight and seed rot disease caused mainly by Burkholderia glumae and Burkholderia gladioli is threatening rice cultivation worldwide. The bacteria have been reported as seed-borne pathogens from rice. Accurate detection of both pathogens on the seeds is very important for limiting the disease dissemination. Novel primer pairs targeting specific molecular markers were developed for the robust detection of B. glumae and B. gladioli. The designed primers were specific in detecting the target species with no apparent crossreactions with other related Burkholderia species at the expected product size. Both primer pairs displayed a high degree of sensitivity for detection of B. glumae and B. gladioli separately in monoplex PCR or simultaneously in duplex PCR from both extracted gDNA and directly preheated bacterial cell suspensions. Limit of detection was as low as 0.1 ng of gDNA of both species and 3.86 × 10² cells for B. glumae and 5.85 × 10² cells for B. gladioli. On inoculated rice seeds, the designed primers could separately or simultaneously detect B. glumae and B. gladioli with a detection limit as low as 1.86 × 10³ cells per rice seed for B. glumae and 1.04 × 10⁴ cells per rice seed of B. gladioli. The novel primers maybe valuable as a more sensitive, specific, and robust tool for the efficient simultaneous detection of B. glumae and B. gladioli on rice seeds, which is important in combating rice panicle blight and seed rot by early detection and confirmation of the dissemination of pathogen-free rice seeds.
목차
Materials and Methods
Results
Discussion
References
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Seed-born Burkholderia glumae Infects Rice Seedling and Maintains Bacterial Population during Vegetative and Reproductive Growth Stage
The Plant Pathology Journal
2018 .10
-
Roles of Quorum Sensing-Dependent Glyoxylate Cycle in Burkholderia glumae
한국미생물학회 학술대회논문집
2015 .04
-
Bio-efficacy of Nanosilicon in Regulating Oxidative Activity to Control Rice Seedlings Rot Disease Caused by Burkholderia glumae
The Plant Pathology Journal
2025 .04
-
Characterization of Burkholderia glumae Putative Virulence Factor 11 (PVF11) via Yeast Two-Hybrid Interaction and Phenotypic Analysis
The Plant Pathology Journal
2019 .06
-
The Roles of Two hfq Genes in the Virulence and Stress Resistance of Burkholderia glumae
The Plant Pathology Journal
2018 .10
-
Stress Tolerance and Virulence-Related Roles of Lipopolysaccharide in Burkholderia glumae
The Plant Pathology Journal
2019 .10
-
High-Throughput Screening of Plant Extracts for Targeted Control of Burkholderia glumae, Causing Rice Sheath and Panicle Blight
The Plant Pathology Journal
2025 .02
-
Simultaneous Detection of Three Bacterial Seed-Borne Diseases in Rice Using Multiplex Polymerase Chain Reaction
The Plant Pathology Journal
2016 .12
-
Seed-to-Seed Transmission and Dynamics of Rice-associated Microbial Communities
한국미생물학회 학술대회논문집
2022 .10
-
Isolation and Characterization of a Novel Bacterium Burkholderia gladioli Bsp-1 Producing Alkaline Lipase
Journal of Microbiology and Biotechnology
2019 .01
-
Growth Prediction of Burkholderia spp. Depending on Temperature
한국미생물학회 학술대회논문집
2022 .10
-
Seed-borne Pathogenic Bacterium Interact with Air-borne Plant Pathogenic Fungus in Rice Fields
한국균학회 학술대회
2018 .01
-
Development of a Selective Medium for Surveillance of Fusarium Head Blight Disease
The Plant Pathology Journal
2024 .04
-
Culturable Endophytes Associated with Soybean Seeds and Their Potential for Suppressing Seed-Borne Pathogens
The Plant Pathology Journal
2022 .08
-
Plant Protective and Growth Promoting Effects of Seed Endophytes in Soybean Plants
The Plant Pathology Journal
2023 .10
-
Efficacy of Diphenyleneiodonium Chloride (DPIC) Against Diverse Plant Pathogens
Mycobiology
2019 .01
-
G-SEED 인증을 받은 공동 주택의 외부 환경 디자인 특성 연구
한국주거학회 학술대회논문집
2020 .11
-
Genome-Wide Analysis of Type VI System Clusters and Effectors in Burkholderia Species
The Plant Pathology Journal
2018 .02
-
Specific and Sensitive Primers Developed by Comparative Genomics to Detect Bacterial Pathogens in Grains
The Plant Pathology Journal
2018 .04
-
Identification of Fungus-infected Tomato Seeds Based on Full-Field Optical Coherence Tomography
Current Optics and Photonics
2019 .12
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0102-2023-481-001815842