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논문 기본 정보

자료유형
학술저널
저자정보
Li-hua Xie (IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture) Xin Quan (IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture) Jie Zhang (IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture) Yan-yan Yang (IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture) Run-hong Sun (IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture) Ming-cong Xia (IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture) Chao Wu (IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture) Xiao-yun Han (IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture) Ya-nan Xue (Agricultural and Animal Husbandry Bureau of Mianchi) and Li-rong Yang (IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture)
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal 제35권 제1호
발행연도
2019.2
수록면
11 - 18 (8page)

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Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. TUBβ was identified as the most stable reference gene. Furthermore, the exopolygalacturonase gene (ExoPG) was selected to verify the reliability of TUBβ expression. The expression profile of ExoPG assessed using TUBβ agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.

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